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Calcium release modulated by inositol trisphosphate in ruptured fibers from frog skeletal muscle

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Abstract

To investigate the effect of inositol 1,4,5-trisphosphate on calcium release, we used fiber bundles of frog sartorius muscle mechanically permeabilized by a scratching procedure, and we detected increments in calcium concentration by measuring aqueorin light signals. Submicromolar concentrations of inositol 1,4,5-trisphosphate induced fast calcium-release signals, with a half time to peak of 60 ms or less. Similar responses were elicited by caffeine. The calcium-release signal induced by inositol 1,4,5-trisphosphate occurred at pCa values of 7 or lower, and the dose-response curve depended on the ionic composition of the incubation solution. Lower inositol 1,4,5-trisphosphate concentrations were needed to induce release when incubation solutions of ionic composition expected to depolarize the transverse tubule membrane were used. Inositol 1,4,5-trisphosphate was more effective than inositol 1,3,4-trisphosphate, inositol 1,4,5,6-tetrakisphosphate, and inositol 1,4-bisphosphate. The effect of inositol 1,4,5-trisphosphate was synergistic with that of caffeine, and was not inhibited by heparin. These results, by showing directly that at resting calcium levels inositol 1,4,5-trisphosphate elicited calcium release, are consistent with a role for inositol 1,4,5-trisphosphate as a chemical modulator in excitation/contraction coupling in skeletal muscle.

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  1. Department of Physiology and Biophysics, Faculty of Medicine, University of Chile, Casilla, 70005, Santiago, Chile

    Cecilia Rojas & Enrique Jaimovich

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  1. Cecilia Rojas
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  2. Enrique Jaimovich
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Rojas, C., Jaimovich, E. Calcium release modulated by inositol trisphosphate in ruptured fibers from frog skeletal muscle. Pflügers Arch 416, 296–304 (1990). https://doi.org/10.1007/BF00392066

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  • DOI: https://doi.org/10.1007/BF00392066

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