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A structural glycoprotein, containing hydroxyproline, isolated from the cell wall of Chlamydomonas reinhardii

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Summary

A soluble extract from purified cell walls of C. reinhardii has been separated by gel filtration into three fractions which together account for 94% of the cell wall. The major fraction (accounting for 70% of the extract) is a glycoprotein, with a molecular wt. in sodium perchlorate of 298,000, which can be split into 4 electrophoretically distinct species. It contains 35% protein with high levels of hydroxyproline, arabinose and galactose, and is capable of self assembly into crystalline structures identical to those found within the cell wall. The second fraction (25% of the extract) is a similar glycoprotein, but contains 24% protein, a higher proportion of mannose, and is incapable of self assembly. The third fraction (3–6% of the extract) is shown to be an adsorbed impurity from the growth medium used.

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Abbreviations

CB or CBB:

coomassie brilliant blue

PAS:

periodic acid Schiff's reagent

PPO:

2,5 diphenyloxazole

PO-POP:

1,4-di[2-(5phenyloxazolyl]benzene

SDS:

sodium dodecyl sulphate

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K.

    J. W. Catt, G. J. Hills & K. Roberts

Authors
  1. J. W. Catt
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  2. G. J. Hills
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  3. K. Roberts
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Additional information

This is the fourth paper in a series entitled ‘Structure, composition and morphogenesis of the cell wall of Chlamydomonas reinhardii. The last paper in this series was Hills et al. (1975)

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Catt, J.W., Hills, G.J. & Roberts, K. A structural glycoprotein, containing hydroxyproline, isolated from the cell wall of Chlamydomonas reinhardii . Planta 131, 165–171 (1976). https://doi.org/10.1007/BF00389990

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  • DOI: https://doi.org/10.1007/BF00389990

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