Abstract
The relationship between temperature and sensitivity to gibberellin A3 (GA3) was studied in lettuce seedlings (Lactuca sativa L. cv. Arctic). Dose/response curves for hypocotyl elongation (10-4 mol l-1 to 10-8 mol l-1) were constructed for a range of temperatures and the slope of the linear portion of the plots used as an indication of the sensitivity to GA3. Hypocotyls were unresponsive to GA3 below 13°C but above this temperature sensitivity increased linearly. Plots of growth rate against temperature had inflexions between 12°C and 13°C, with slopes above this point which increased with increasing GA3 concentration. The Q10 value for response increased in a similar manner. Reaction rates of NAD-dependent malate dehydrogenase and peroxidase extracted from hypocotyls varied linearly with temperature whilst nonspecific tetrazolium reduction, a membrane based activity, showed an abrupt rate change above 14°C. Pre-exposure to GA3 had no effect on the temperature responses of soluble or particulate enzymes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- GA3 :
-
gibberellin A3
References
Hedley, C.L., Stoddart, J.L.: Factors influencing alanine amino-transferase activity in leaves of Lolium temulentum L. I. Photoperiodically induced variations. J. exp. Bot. 22, 239–248 (1971)
Jones, T.W.A., Stoddart, J.L.: Gibberellin-induced changes in protein synthesis and enzyme activity in shoot apices of Trifolium pratense. J. exp. Bot. 21, 452–461 (1970)
Lyons, J.M., Raison, J.K.: Oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury. Plant Physiol. 45, 386–389 (1970)
Raison, J.K.: Temperature-induced phase changes in membrane lipids and their influence on metabolic regulation. Symp. Soc. Exp. Biol. 27, 485–512 (1973)
Silk, W.K., Jones, R.L.: Gibberellin response in lettuce hypocotyl sections. Plant Physiol. 56, 267–272 (1975)
Silk, W.K., Stoddart, J.L., Jones, R.L.: Growth and gibberellin A1 metabolism in excised lettuce hypocotyls. Plant Physiol. 59, 211–216 (1977)
Steponkus, P.L., Lanphear, F.O.: Refinement of the triphenyl tetrazolium chloride method of determining cold injury. Plant Physiol. 42, 1423–1426 (1967)
Stuart, D.A., Jones, R.L.: Roles of extensibility and turgor in gibberellin- and dark-stimulated growth. Plant Physiol. 59, 61–68 (1977)
Wood, A., Paleg, L.G.: The influence of gibberellic acid on the permeability of model membrane systems. Plant Physiol. 50, 103–108 (1972)
Wood, A., Paleg, L.G.: Alteration of liposomal membrane fluidity by gibberellic acid. Aust. J. Plant Physiol. 1, 31–40 (1974)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stoddart, J.L., Tapster, S.M. & Jones, T.W.A. Temperature dependence of the gibberellin response in lettuce hypocotyls. Planta 141, 283–288 (1978). https://doi.org/10.1007/BF00388345
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388345