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A deficiency of hypoxanthine phosphoribosyltransferase in a sycamore callus resistant to azaguanine

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Summary

Sycamore (Acer pseudoplatanus L.) calluses resistant to azaguanine were isolated after mutagenesis with nitrosoguanidine. One callus SNC61 was highly variable in its growth on 10 μg/ml azaguanine and it was concluded that its resistance was not due to a stable alteration in the callus. Another callus SNE31 was isolated after 4.5 months culture in 0.5 or 1 μg/ml azaguanine which had a 10-fold increase in resistance to azaguanine. This was shown to be accompanied by a reduction to 50% of the normal level of hypoxanthine phosphoribosyltransferase (E.C.2.4.2.8).

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Abbreviations

HAT:

hypoxanthine-aminopterin-thymidine system

GM:

medium of Gamborg

HPRT:

hypoxanthine phosphoribosyltransferase

References

  • Bright, S. W. J., Northcote, D. H.: Protoplast regeneration from normal and bromodeoxyuridine-resistant sycamore callus. J. Cell Sci. 16, 445–464 (1974)

    Google Scholar 

  • Brockman, R. W., Debavadi, C. S., Stutts P., Hutchinson, D. J.: Purine ribonucleotide pyrophosphorylases and resistance to purine analogues in Streptococcus faecalis. J. biol. Chem. 236, 1471–1479 (1961)

    Google Scholar 

  • Carlson, P. S.: Production of auxotrophic mutants in ferns. Genet. Res. 14, 337–339 (1969)

    Google Scholar 

  • Carlson, P. S.: Induction and isolation of auxotrophic mutants in somatic cell cultures of Nicotiana tabacum. Science 168, 487–489 (1970)

    Google Scholar 

  • Carlson, P. S., Smith, H. H., Dearing, R. D.: Parasexual interspecific plant hybridization. Proc. nat. Acad. Sci. (Wash.) 69, 2292–2294 (1972)

    Google Scholar 

  • Chu, E. H. Y., Malling, H. V.: Mammalian cell genetics II. Chemical induction of specific locus mutations in chinese hamster cells in vitro. Proc. nat. Acad. Sci. (Wash.) 61, 1306–1312 (1968)

    Google Scholar 

  • Dawson, R. M. C., Elliott, D. C., Elliott, W. H., Jones, K. M.: In data for biochemical research, p. 619. London: Oxford Univ. Press 1969

    Google Scholar 

  • Foster, R. C.: Chromosome numbers in Acer and Staphylea. J. Arn. Arb. 14, 386–393 (1933)

    Google Scholar 

  • Gamborg, O. L.: Aromatic metabolism in plants II. Enzymes of the shikimate pathway in suspension cultures of plant cells. Canad. J. Biochem. 44, 791–799 (1966)

    Google Scholar 

  • Harris, H., Cook, P. R.: Synthesis of an enzyme determined by an erythrocyte nucleus in a hybrid cell. J. Cell Sci. 5, 121–133 (1969)

    Google Scholar 

  • Harris, M.: Mutation rates in cells at different ploidy levels. J. cell. Physiol. 78, 177–184 (1971)

    Google Scholar 

  • Hill, H. M., Rogers, L. J.: Mevalonate activating enzymes and phosphatases in higher plants. Phytochem. 13, 763–777 (1974)

    Google Scholar 

  • Kao, K. M., Michayluk, M. R.: A method for high frequency intergeneric fusion of plant protoplasts. Planta (Berl.) 115, 355–367 (1974)

    Google Scholar 

  • Keller, W. A., Melchers, G.: The effect of high pH and calcium on tobacco leaf protoplast fusion. Z. Naturforsch. 28 c, 737–741 (1973)

    Google Scholar 

  • Klinger, H. P., Shin, S. I.: Modulation of the activity of an avian gene transferred into a mammalian cell by cell fusion. Proc. nat. Acad. Sci. (Wash.) 71, 1398–1402 (1974)

    Google Scholar 

  • Lescure, A. M.: Selection of markers of resistance to base analogues in somatic cell cultures of Nicotiana tabacum. Pl. Sci. Lett. 1, 375–383 (1973)

    Google Scholar 

  • Littlefield, J. W.: The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. nat. Acad. Sci. (Wash.) 50, 568–576 (1963)

    Google Scholar 

  • Littlefield, J. W.: Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145, 709–710 (1964a)

    Google Scholar 

  • Littlefield, J. W.: Three degrees of guanylic acid-inosinic acid pyrophosphorylase deficiency in mouse fibroblasts. Nature (Lond.) 203, 1142–1144 (1964b)

    Google Scholar 

  • Littlefield, J. W., Basilico, C.: Infection of thymidine kinase-deficient BHK cells with polyoma virus. Nature (Lond.) 211, 250–252 (1966)

    Google Scholar 

  • Mackenzie, I. A., Konar, A., Street, H. E.: Cytokinins and the growth of cultured sycamore cells. New Phytol. 71, 633–638 (1972)

    Google Scholar 

  • Maliga, P., Marton, L., Sz.Breznovits A.: 5-bromodeoxyuridine-resistant cell lines from haploid tobacco. Pl. Sci. Lett. 1, 119–121 (1973)

    Google Scholar 

  • Orkin, S. H., Littlefield, J. W.: Nitrosoguanidine mutagenesis in synchronised hamster cells. Exp. Cell Res. 66, 69–74 (1970)

    Google Scholar 

  • Riccardi, V. M., Littlefield, J. W.: Adaptation in Lesch-Nyhan cells exposed to aminopterin. Exp. Cell Res. 74, 417–422 (1972)

    Google Scholar 

  • Shin, S. I.: Nature of mutations conferring resistance to 8-azaguanine in mouse cell lines. J. Cell Sci. 14, 235–251 (1974)

    Google Scholar 

  • Stuart, R., Street, H. E.: Studies on the growth in culture of plant cells. X. Further studies in the conditioning of culture media by suspensions of Acer pseudoplatanus L. cells. J. exp. Bot. 22, 96–106 (1971)

    Google Scholar 

  • Szybalski W., Szybalska, E. H., Ragni, G.: Genetic studies with human cell lines. Nat. Cancer Inst. Monogr. 7, 75–89 (1962)

    Google Scholar 

  • Webb, J. L.: Enzyme and metabolic inhibitors, vol. 2, p. 477–478. New York: Academic Press 1966

    Google Scholar 

  • Widholm, J. M.: Tryptophan biosynthesis in Nicotiana tabacum and Daucus carota cell cultures: Site of action of inhibitory tryptophan analogues. Biochim. biophys. Acta (Amst.) 261, 44–51 (1972a)

    Google Scholar 

  • Widholm, J. M.: Cultured Nicotiana tabacum cells with an altered anthranilate synthetase which is less sensitive to feedback inhibition. Biochim. biophys. Acta (Amst.) 261, 52–58 (1972b)

    Google Scholar 

  • Wright, K., Northcote, D. H.: Induced root differentiation in sycamore callus. J. Cell Sci. 11, 319–337 (1972)

    Google Scholar 

  • Wright, K., Northcote, D. H.: Differences in ploidy and degree of intercellular contact in differentiating and non-differentiating sycamore calluses. J. Cell Sci. 12, 37–53 (1973)

    Google Scholar 

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Bright, S.W.J., Northcote, D.H. A deficiency of hypoxanthine phosphoribosyltransferase in a sycamore callus resistant to azaguanine. Planta 123, 79–89 (1975). https://doi.org/10.1007/BF00388062

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  • DOI: https://doi.org/10.1007/BF00388062

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