Summary
Sycamore (Acer pseudoplatanus L.) calluses resistant to azaguanine were isolated after mutagenesis with nitrosoguanidine. One callus SNC61 was highly variable in its growth on 10 μg/ml azaguanine and it was concluded that its resistance was not due to a stable alteration in the callus. Another callus SNE31 was isolated after 4.5 months culture in 0.5 or 1 μg/ml azaguanine which had a 10-fold increase in resistance to azaguanine. This was shown to be accompanied by a reduction to 50% of the normal level of hypoxanthine phosphoribosyltransferase (E.C.2.4.2.8).
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Abbreviations
- HAT:
-
hypoxanthine-aminopterin-thymidine system
- GM:
-
medium of Gamborg
- HPRT:
-
hypoxanthine phosphoribosyltransferase
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Bright, S.W.J., Northcote, D.H. A deficiency of hypoxanthine phosphoribosyltransferase in a sycamore callus resistant to azaguanine. Planta 123, 79–89 (1975). https://doi.org/10.1007/BF00388062
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DOI: https://doi.org/10.1007/BF00388062