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Detection and preliminary identification of endogenous antitranspirants in water-stressed Sorghum plants

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Summary

Sorghum plants that had been subjected to different degrees of water stress were examined for the occurrence of endogenous compounds capable of inducing stomatal closure, i.e. “antitranspirants”. Acidic extracts contained increased amounts of abscisic acid (ABA) as the amount of stress increased, but another highly active compound easily distinguished from ABA also accumulated. This compound, also found in neutral extracts, was probably all trans-farnesol, an isoprenoid alcohol which, like ABA, is a sesquiterpenoid. Highly dilute solutions of “commercial” farnesol induced stomatal closure when applied to isolated epidermis of Commelina.

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References

  • Allaway, W.G., Mansfield, T.A.: Experiments and observations on the after effect of wilting on stomata of Rumex sanguineus. Canad. J. Bot. 48, 513–521 (1970)

    Google Scholar 

  • Burton, W.G.: Some observations on the growth substances in ether extracts of the potato tuber. Physiol. Plantarum (Cph.) 9, 567–587 (1956)

    Google Scholar 

  • Davis, L.A., Heinz, D.E., Addicott, F.T.: Gas-liquid chromatography of trimethylsilyl derivatives of abscisic acid and other plant hormones. Plant Physiol. 43, 1389–1394 (1968)

    Google Scholar 

  • De Boer, T.J., Backer, H.J.: A new method for the preparation of diazomethane. Rec. Trav. chim. Pays-Bas 73, 229–234 (1954)

    Google Scholar 

  • Dunphy, P.J., Kerr, J.D., Pennock, J.F., Whittle, K.: A simple method for the identification and separation of isoperenoid alcohols. Chemistry and Industry 37, 1549–1550 (1966)

    Google Scholar 

  • Fischer, R.A.: After-effect of water stress on stomatal opening potential. II. Possible causes. J. exp. Bot. 21, 386–404

  • Heath, O.V.S., Mansfield, T.A.: A recording porometer with detachable cups operating on four separate leaves. Proc. roy. Soc. B. 156, 1–13 (1962)

    Google Scholar 

  • Jones, R.J., Mansfield, T.A.: Suppresiion of stomatal opening in leaves treated with abscisic acid. J. exp. Bot. 21, 714–719 (1970)

    Google Scholar 

  • Laidman, D.L., Morton, R.A., Paterson, J.Y.F., Pennock, J.F.: Substance SC (Ubichromenol): A naturally-occurring cyclic isomeride of ubiquinone-50. Biochem. J. 74, 541–549 (1960)

    PubMed  Google Scholar 

  • McWha, J.A., Philipson, J.J., Hillman, J.R., Wilkins, M.B.: Molecular requirements for abscisic acid activity in two bioassay systems. Planta (Berl.) 109, 327–336 (1973)

    Google Scholar 

  • Milborrow, B.V.: Biosynthesis of abscisic acid and its regulation. Abstracts, 8th Int. Conf. Plant growth substances, 97 (1973)

  • Mizrahi, Y., Blumenfield, A., Richmond, A.E.: Abscisic acid and transpiration in leaves in relation to osmotic root stress. Plant Physiol. 46, 169–171 (1970)

    Google Scholar 

  • Most, B.H.: Abscisic acid in immature apical tissue of sugar cane and in leaves of plants subjected to drought. Planta (Berl.) 101, 67–75 (1971)

    Google Scholar 

  • Nitsch, J.P., Nitsch, C.: Studies on the growth of coleoptile and first internode sections. Plant Physiol. 31, 44–111 (1956)

    Google Scholar 

  • Ogunkanmi, A.B., Tucker, D.J., Mansfield, T.A.: An improved bioassay for abscisic acid and other antitranspirants. New Phytol. 72, 277–282 (1973)

    Google Scholar 

  • Schlenk, H., Gellerman, J.L.: Esterification of fatty acids with diazomethane on a small scale. Analyt. Chem. 32, 1412–1414 (1960)

    Google Scholar 

  • Sondheimer, E., Walton, D.C.: Structure-activity correlations with compounds related to abscisic acid. Plant Physiol. 45, 244–248 (1970)

    PubMed  Google Scholar 

  • Tucker, D.J., Mansfield, T.A.: A simple bioassay for detecting antitranspirant activity of naturally occurring compounds such as abscisic acid. Planta (Berl.) 98, 157–163 (1971)

    Google Scholar 

  • Wellburn, A.R.: The stereochemistry of hydrogen transfer during the reduction of C-20 isoprenoids in plants. Phytochemistry 7, 1523–1528 (1968)

    Google Scholar 

  • Wright, S.T.C.: An increase in the inhibitor-β content of detached wheat leaves following a period of wilting. Planta (Berl.) 86, 10–20 (1969)

    Google Scholar 

  • Wright, S.T.C., Hiron, R.W.P.: (+)-abscisic acid, the growth inhibitor in detached wheat leaves following a period of wilting. Nature (Lond.) 224, 719–720 (1969)

    Google Scholar 

  • Zeevaart, J.A.D.: (+)-abscisic acid content of spinach in relation to photoperiod and water stress. Plant Physiol. 48, 86–90 (1971)

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, University of Lancaster, LA1 4YQ, Lancaster, UK

    A. B. Ogunkanmi, A. R. Wellbern & T. A. Mansfield

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  1. A. B. Ogunkanmi
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  2. A. R. Wellbern
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  3. T. A. Mansfield
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Ogunkanmi, A.B., Wellbern, A.R. & Mansfield, T.A. Detection and preliminary identification of endogenous antitranspirants in water-stressed Sorghum plants. Planta 117, 293–302 (1974). https://doi.org/10.1007/BF00388024

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  • DOI: https://doi.org/10.1007/BF00388024

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