Summary
Some of the physiological and biochemical changes which were found to occur during paraquat (1,1′-dimethyl-4,4′-bipyridylium ion) treatment of cotyledon leaves of Linium usitatissimum, are reported. Results showed an inhibition of photosynthetic CO2 uptake and electron flow of isolated chloroplasts. An increase in membrane permeability, changes in the level of the major lipid components and of malondialdehyde. These are correlated with ultrastructural changes and the discussion includes a proposed mode of action for the herbicides.
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References
Akhavein, A. A., Linscott, D. L.: The dipyridylium herbicides, paraquat and diquat. Residue Rev. 23, 97–145 (1968).
Anderson, I. C., Robertson, D. S.: Role of carotenoids in protecting chlorophyll from photodestruction. Plant Physiol. 35, 531–534 (1960).
Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949).
Baddiley, J., Buchanan, J. G., Handschumaker, R. E., Prescott, J. F.: Chemical studies in the biosynthesis of purine nucleotides I. The preparation of N-glycylglycosylamines. J. chem. Soc. 2818–2823 (1956).
Baldwin, B. C., Dodge, A. D., Harris, N.: Recent advances in studies of the mode of action of the bipyridylium herbicides. Proc. 9th Br. Weed Control Conf. 639–644 (1968).
Black, C. C., Myers, L.: Some biochemical aspects of the mechanisms of herbicidal activity. Weeds 14, 331–338 (1966).
Bligh, E. G., Dyer, W. J.: A rapid method of total lipid extraction and purification. Canad. J. Biochem. Physiol. 37, 911–917 (1959).
Brian, R. C.: Darkness and the activity of diquat and paraquat on tomato, broad bean and sugar beet. Ann. appl. Biol. 60, 77–85 (1967).
Brundish, D. E., Shaw, N., Baddiley, J.: The structure and possible function of the glycolipid from Staphylococcus lactis 13. Biochem. J. 105, 885–889 (1967).
Calderbank, A.: The bibyridylium herbicides. Advanc. Pest Control Res. 8, 127–235 (1968).
Davenport, H. E.: The mechanism of cyclic phosphorylation by illuminated chloroplasts. Proc. roy. Soc. B 157, 332–345 (1963).
Eilam, Y.: Permeability changes in senescing tissue. J. exp. Bot. 16, 614–627 (1965).
Gregory, R. P. F.: An improved preparative method for spinach catalase and evaluation of some of its properties. Biochim. biophys. Acta (Amst.) 159, 429–439 (1968).
Harris, N., Dodge, A. D.: The effect of paraquat on flax cotyledon leaves: Changes in fine structure. Planta (Berl.) 104, 201–209 (1972).
Heath, R. L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stochiometry of fatty acid peroxidation. Arch. Biochem. 125, 189–198 (1968).
Hill, R., Walker, D. A.: Pyocyanine and phosphorylation with chloroplasts. Plant Physiol. 34, 240–245 (1969).
Jagendorf, A. T., Margulies, M. M.: Inhibition of spinach chloroplast photochemical reactions by p-chlorophenyl 1-1, dimethyl urea. Arch. Biochem. 90, 184–195 (1960).
Kirk, J. T. O., Allen, R. L.: Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochem. biophys. Res. Commun. 21, 523–530 (1965).
Kohn, H., Liversedge, M.: On a new aerobic metabolite whose production by brain is inhibited by amorphine, emetrine, ergotamine, epinephrine and menadione. J. Pharmacol. exp. Ther. 82, 292–300 (1944).
Koski, V. M., Smith, J. H. C.: Chlorophyll formation in a mutant, white seeding. Arch. Biochem. 34, 189–195 (1951).
Marinetti, G. V., Erbland, J., Stotz, E.: Phosphatides of pig heart cell fractions. J. biol. Chem. 233, 562–565 (1958).
Matile, Ph.: Enzyme der Vakuolen und Wurzelzellen von Maiskeimlingen. Ein Beitrag zur funktionellen Bedeutung der Vakuole bei der intrazellularen Verdauung. Z. Naturforsch. 21b, 871–878 (1966).
Mees, G. C.: Experiments on the herbicidal action of 1,1-ethylene-2,2-dipyridylium dibromide. Ann. appl. Biol. 48, 601–612 (1960).
Neish, A. C.: Studies on chloroplasts II. Their chemical composition and distribution of certain metabolites between the chloroplasts and the remainder of the leaf. Biochem. J. 33, 300–308 (1939).
Patton, S., Kurtz, G.: 2-Thiobarbituric acid as a reagent for detecting milk fat oxidation. J. Dairy Sci. 34, 669–674 (1951).
Skidmore, N. D., Entenman, C.: Two dimensional thin layer chromatography of rat liver phosphatides. J. Lipid Res. 3, 471–475 (1962).
Tolbert, N. E., Oeser, A., Kisaki, T., Hageman, R. H., Yamazaki, R. K.: Peroxisomes from spinach leaves containing enzymes related to glycolate metabolism. J. biol. Chem. 243, 5179–5184 (1962).
Vernon, L. P.: Spectrophotometric determination of chlorophylls and phaeophytins in plant extracts. Analyt. Chem. 32, 1144–1150 (1960).
Yamazaki, R. K., Tolbert, N. E.: Enzymic characterization of leaf peroxisomes. J. biol. Chem. 245, 5137–5144 (1970).
Zweig, G., Shavit, N., Avron, M.: Diquat (1,1-ethylene-2,2-dipyridylium dibromide) in photoreactions of isolated chloroplasts. Biochim. biophys. Acta (Amst.) 109, 332–346 (1965).
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Harris, N., Dodge, A.D. The effect of paraquat on flax cotyledon leaves: Physiological and biochemical changes. Planta 104, 210–219 (1972). https://doi.org/10.1007/BF00387076
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DOI: https://doi.org/10.1007/BF00387076