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The mechanism of hydrogen photoproduction by several algae

II. The contribution of photosystem II

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Summary

The contribution of PS II to H2 photoproduction by several unicellular green algae was measured both when O2 evolution and photophosphorylation were unimpaired and also when these processes had been eliminated by Cl-CCP. As judged by the effects of DCMU, a PS II contribution was found under both sets of experimental conditions for several strains of Chlorella, Ankistrodesmus and Scenedesmus. However, H2 photoproduction by Chlamydomonas moewusii was insensitive to DCMU and thus was entirely due to PS I. With cells treated with Cl-CCP, the relative amount of PS II contribution varied from zero in autotrophically grown Chlamydomonas reinhardii, to ≈ 20% in photoheterotrophically grown and ≈ 50% in autotrophically grown cells of Ankistrodesmus braunii, Chlorella fusca, Chlorella vulgaris and Scenedesmus obliquus. The dehydrogenation of reduced H-donors by PS II of Scenedesmus treated with Cl-CCP showed the same biphasic kinetics previously described for H2 photoproduction by PS I of this alga.

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Abbreviations

Cl-CCP:

carbonyl cyanide m-chlorophenylhydrazone

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

ICC:

Indiana Culture Collection

PS:

photosystem

SAL:

salicylaldoxime

SIO:

Marine Botany Culture Collection, Scripps Institution of Oceanography

References

  • Avron, M.: Mechanism of photoinduced electron transport in isolated chloroplasts. In: Current topics in bioenergetics, vol. II, p. 1–22 (D. R. Sanadi, ed.). New York: Acad. Press (1967).

    Google Scholar 

  • Bishop, N. I.: partial reactions of photosynthesis and photoreduction. Ann. Rev. Plant Physiol. 17, 185–208 (1966).

    Google Scholar 

  • Bishop, N. I.: Photosynthesis: the electron transport system of green plants. Ann. Rev. Biochem. 40, 197–226 (1971).

    Google Scholar 

  • Bishop, N. I., Gaffron, H.: On the interrelation of the mechanisms for oxygen and hydrogen evolution in adapted algae. In: Photosynthetic mechanisms in green plants. Publ. No 1145, Nat. Acad. Sci.-Nat. Res. Counc., p. 441–451 (1963).

  • Boardman, N. K.: Physical separation of the photosynthetic photochemical systems. Ann. Rev. Plant Physiol. 21, 115–140 (1970).

    Google Scholar 

  • Cheniae, G. M.: Photosystem II and O2 evolution. Ann. Rev. Plant Physiol. 21, 467–498 (1970).

    Google Scholar 

  • Cheniae, G. M., Martin, I. F.: Photoreactivation of manganese catalyst in photosynthetic oxygen evolution. Plant Physiol. 44, 351–360 (1969).

    Google Scholar 

  • Duysens, L. N. M., Sweers, H. E.: Mechanisms of two photochemical reactions in algae as studied by means of fluorescence. In: Studies on microalgae and photosynthetic bacteria, p. 353–372. Tokyo: Japanese Soc. Plant Physiologists 1963.

    Google Scholar 

  • Forbush, B., Kok, B.: Reaction between primary and secondary electron acceptors of photosystem II of photosynthesis. Biochim. biophys. Acta (Amst.) 162, 243–253 (1968).

    Google Scholar 

  • Fork, D. C., Amesz, J.: Action spectra and energy transfer in photosynthesis. Ann. Rev. Plant Physiol. 20, 305–328 (1969).

    Google Scholar 

  • Gaffron, H., Rubin, J.: Fermentative and photochemical production of hydrogen in algae. J. gen. Physiol. 26, 219–240 (1942).

    Google Scholar 

  • Gingras, G., Lavorel, J.: Proprietés de fluorescence de Scenedesmus adapté a l'hydrogène. Physiol. Veget. 3, 109–120 (1965).

    Google Scholar 

  • Healey, F. P.: Hydrogen evolution by several algae. Planta (Berl.) 91, 220–226 (1970a).

    Google Scholar 

  • Healey, F. P.: The mechanism of hydrogen evolution by Chlamydomonas moewusii. Plant Physiol. 45, 153–159 (1970b).

    Google Scholar 

  • Hind, G., Olson, J. M.: Electron transport pathways in photosynthesis. Ann. Rev. Plant Physiol. 19, 249–282 (1968).

    Google Scholar 

  • Homann, P. H.: Fluorescence properties of chloroplasts from manganese deficient and mutant tobacco. Biochim. biophys. Acta (Amst.) 162, 545–554 (1968).

    Google Scholar 

  • Homann, P. H.: Actions of carbonylcyanide m-chlorophenylhydrazone on electron transport and fluorescence of isolated chloroplasts. Biochim. biophys. Acta (Amst.) 245, 129–143 (1971).

    Google Scholar 

  • Inoué, H., Wakamatsu, K., Nishimura, M.: Light-induced absorbance changes at 475–515 nm, and electron flow close to photosystem II in Tris-washed chloroplast fragments. Plant Cell Physiol. 12, 457–460 (1971).

    Google Scholar 

  • Itoh, M., Yamashita, K., Nishi, T., Konishi, K., Shibata, K.: The site of manganese function in photosynthetic electron transport system. Biochim. biophys. Acta (Amst.) 180, 509–519 (1969).

    Google Scholar 

  • Izawa, S.: Effect of Hill reaction inhibitors on photosystem I. In: Comparative biochemistry and biophysics of photosynthesis, p. 140–147 (K. Shibata, A. Takamiya, A. T. Jagendorf and R. C. Fuller, eds.). Tokyo: Univ. Tokyo Press 1968.

    Google Scholar 

  • Kaltwasser, H., Stuart, T. S., Gaffron, H.: Light-dependent hydrogen evolution by Scenedesmus. Planta (Berl.) 89, 309–322 (1969).

    Google Scholar 

  • Katoh, S., San Pietro, A.: Ascorbate-supported NADP photoreduction by heated Euglena chloroplasts. Arch. Biochem. 122, 144–152 (1967).

    Google Scholar 

  • Kessler, E.: Effect of hydrogen adaptation on fluorescence in normal and manganese-deficient algae. Planta (Berl.) 81, 264–273 (1968).

    Google Scholar 

  • Kessler, E.: Photosynthesis, photooxidation of chlorophyll and fluorescence of normal and manganese-deficient Chlorella with and without hydrogenase. Planta (Berl.) 92, 222–234 (1970).

    Google Scholar 

  • Kiewiet, D. Y. de, Hall, D. O., Jenner, E. L.: Effect of carbonylcyanide m-chlorophenylhydrazone on the photochemical reactions of isolated chloroplasts. Biochim. biophys. Acta (Amst.) 109, 284–292 (1965).

    Google Scholar 

  • Kimimura, M., Katoh, S., Ikegami, I., Takamiya, A.: Inhibitory site of carbonyl cyanide m-chlorophenylhydrazone in the electron transfer system of chloroplasts. Biochim. biophys. Acta (Amst.) 234, 92–102 (1971).

    Google Scholar 

  • Malkin, S., Kok, B.: Fluorescence induction studies in isolated chloroplasts. I. Number of components involved in the reaction and quantum yields. Biochim. biophys. Acta (Amst.) 126, 413–432 (1966).

    Google Scholar 

  • Spruit, C. J. P.: Simultaneous photoproduction of hydrogen and oxygen by Chlorella. Meded. Landbouwhogeschool Wageningen 58, Nr. 9, 1–17 (1958).

    Google Scholar 

  • Stuart, T. S.: Hydrogen production by photosystem I of Scenedesmus: Effect of heat and salicylaldoxime on electron transport and photophosphorylation. Planta (Berl.) 96, 81–92 (1971).

    Google Scholar 

  • Stuart, T. S., Gaffron, H.: The kinetics of hydrogen photoproduction by adapted Scenedesmus. Planta (Berl.) 100, 228–243 (1971).

    Google Scholar 

  • Stuart, T. S., Gaffron, H.: The gas exchange of hydrogen-adapted algae as followed by mass spectrometry. Plant Physiol., in press (1972a).

  • Stuart, T. S., Gaffron, H.: The mechanism of hydrogen photoproduction by several algae. I. The effect of inhibitors of photophosphorylation. Planta (Berl.) 106, 91–100 (1972b).

    Google Scholar 

  • Stuart, T. S., Herold, E. W., Gaffron, H.: A simple combination mass spectrometer inlet and oxygen electrode chamber for sampling gases dissolved in liquids. Analyt. Biochem. 46, 91–100 (1972).

    PubMed  Google Scholar 

  • Stuart, T. S., Kaltwasser, H.: Photoproduction of hydrogen by photosystem I of Scenedesmus. Planta (Berl.) 91, 302–312 (1970).

    Google Scholar 

  • Yamashita, K., Konishi, K., Itoh, M., Shibata, K.: Photo-bleaching of carotenoids related to the electron transport in chloroplasts. Biochim. biophys. Acta (Amst.) 172, 511–524 (1969).

    Google Scholar 

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Authors and Affiliations

  1. Institute of Molecular Biophysics and Department of Biological Science, Florida State University, Tallahassee, USA

    Tim S. Stuart & Hans Gaffron

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  1. Tim S. Stuart
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  2. Hans Gaffron
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Additional information

These studies were supported by contract No. AT-(40-1)-2687 from the U.S. Atomic Energy Commission.

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Stuart, T.S., Gaffron, H. The mechanism of hydrogen photoproduction by several algae. Planta 106, 101–112 (1972). https://doi.org/10.1007/BF00383990

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  • DOI: https://doi.org/10.1007/BF00383990

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