Abstract
Vacuoles were isolated from red beets (Beta vulgaris L.) by slicing the tissue and separated using a discontinuous dextran gradient centrifugation. The uptake of sucrose against a concentration gradient into the dextran-impermeable [3H]-H2O space of these organelles was studied using silicone layer filtering centrifugation on both fluorometric and 14C-measurement of sucrose. The rate is 24 nmol sucrose (unit betacyanin)-1 h-1 and appears to be stimulated by ATP to an uptake rate of 34 nmol. Control experiments with slices cut from red beet tissue and incubated with [14C]sucrose gave comparable results. An ATPase activity dependent on both Mg2+ and K+ seems to be localized at the inner surface of the tonoplast. This activity is strongly inhibited by EDAC and tartrate and there is no effect of oligomycin, whereas a slight stimulation was caused by DCCD.
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Abbreviations
- CCCP:
-
carbonylcyanide-m-chlorophenylhydrazone
- EDAC:
-
ethyl-3(3-dimethylaminopropylcarbodiimide)
- EDTA:
-
ethylenediamine tetraacetic acid
- fr.wt.:
-
fresh weight
- HEPES:
-
n-2-hydroxyethylepiperazine-n′-2-ethanesulfonic acid
- MES:
-
2(n-morpholino)ethane sulfonic acid
- Pi :
-
inorganic phosphate
- Tris:
-
tris-(hydroxymethyl)-aminomethan
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Dedicated to A.L. Kursanov, Moscow, on his 75th birhday
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Doll, S., Rodier, F. & Willenbrink, J. Accumulation of sucrose in vacuoles isolated from red beet tissue. Planta 144, 407–411 (1979). https://doi.org/10.1007/BF00380115
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DOI: https://doi.org/10.1007/BF00380115