Abstract
Urethane foam was applied to immobilize the fungus Penicillium chrysogenum in order to accelerate penicillin fermentation. Various operational conditions, such as cultivation temperature, initial pH value, composition of substrate and inoculum size of spores, which might influence penicillin fermentation significantly, were studied in a shaking flask culture system.
The results are summarized as follows:
-
1)
The maximum production amount of penicillin was achieved when cultivation temperature and initial pH were kept at 25±1°C and 4, respectively.
-
2)
The maximum production yield of penicillin for substrate was obtainable when the concentration of lactose and that of cornsteep liquor in the basal medium were adjusted at 60 kg/m3 and 30 kg/m3, respectively.
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3)
It was found in this cultivation system that the optimum region concerning inoculum size was enlarged from 7.0·105 to 1.0·107 spores/dm3 while that of the traditional mehtod was very narrow around 1.0·107 spores/dm3. Moreover, the production amount of penicillin produced by this new method was about twelve times as high as that produced by the traditional method.
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Abbreviations
- C 0 kg/m3 :
-
initial concentration of cornsteep liquor
- d p mm:
-
mean diameter of mycelial pellets
- L kg/m3 :
-
lactose concentration
- L 0 kg/m3 :
-
initial concentration of lactose
- l A mm:
-
mean thickness of mycelial layer on the surface of cubic urethane foams
- N 0 spores/dm3 :
-
inoculum concentration of spores
- P kg/m3 :
-
penicillin concentration
- T °C:
-
cultivation temperature
- X kg/m3 :
-
dry cell mass concentration of mycelia
- m :
-
maximum values
- *:
-
dimensionless values
References
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Deo, Y. M.; Gaucher, G. M.: Semicontinous and continous production of Penicillin-G by Penicillium chrysogenum cells immobilized in ϰ-Carrageenan beads. Biotechnol. Bioeng. 26 (1984) 285–295
Endo, I.; Nagamune, T.; Kato, N.; Nishimura, M.; Kobayashi, T.: A new cultivation method of fungi or mycelia. Bioprocess Eng 3 (1988) 63–68
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Nagamune, T., Endo, I., Kato, N. et al. The effect of cultivation conditions on the penicillin production using a urethane foam-supported Penicillium chrysogenum. Bioprocess Engineering 3, 173–176 (1988). https://doi.org/10.1007/BF00379653
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DOI: https://doi.org/10.1007/BF00379653