Abstract
A monoclonal antibody, MHM4, identified a cell surface antigen present on B cells and not resting T cells. It precipitated two polypeptide chains of 34 000 and 28 000 daltons from B lymphoblastoid cells. This antibody bound to all B-cell lines tested, except those homozygous for HLA-DR7. Saturation binding assays and Scatchard plots of MHM4 binding to cells that did not carry HLA-DR7 indicated that this antibody bound less than the total surface Ia antigen. When the antibody was competed with eight other HLA-D-specific antibodies, the epitope recognized was shown to be distinct. Two-dimensional gel analysis revealed that a simple pattern of spots was precipitated, unlike the complex patterns obtained with other HLA-D-specific antibodies. The α and β spots were different from those precipitated by HLA-DR- or DC-specific antibodies. It is argued that the MHM4 antigen is the product of an HLA locus that is distinct from HLA-DR and DC. Its relationship with HLA-SI3 is discussed.
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Abbreviations
- BSA:
-
bovine serum albumin
- DEAF:
-
dimethylaminoethyl
- DOC:
-
sodium deoxycholate
- GLO:
-
glyoxylase
- 2-ME:
-
2 mercaptoethanol
- PAGE:
-
polyacrylamide gel electrophoresis
- PBL:
-
peripheral blood lymphocytes
- PBS:
-
phosphate-buffered saline
- NEPHGE:
-
nonequilibrium pH gradient electrophoresis
- NMS:
-
normal mouse serum
- NP40:
-
nonidet p40
- RAM:
-
rabbit anti-mouse immunoglobulin
- RAMIgG:
-
rabbit anti-mouse immunoglobulinG
- SDS:
-
sodium dodecyl sulphate
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Makgobat, M.W., Hildreth, J.E.K. & McMichael, A.J. Identification of a human Ia antigen that is different from HLA-DR and DC antigens. Immunogenetics 17, 623–635 (1983). https://doi.org/10.1007/BF00366130
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DOI: https://doi.org/10.1007/BF00366130