Summary
YEp plasmid stability in the presence of either Saccharomyces cerevisiae laboratory strain 2-μm DNA, or lager brewing yeast 2-μm DNA in the same genetic background, was compared under non-selective culture conditions. It was found that YEp plasmids were more stably maintained in the presence of lager 2-μm DNA under these conditions. By construction of laboratory-lager 2-μm DNA hybrid plasmids, an 867 bp StuI fragment of lager 2-μm DNA was shown to be responsible for the enhanced stability of the YEp plasmid. Nucleotide substitutions at two sites were found by sequencing this region. It was also confirmed that increasing cell ploidy enhanced YEp stability under non-selective conditions.
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Communicated by A. Hinnen
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Hosford, E.A., Sone, H. & Tanaka, Ji. Enhanced stability of YEp plasmids in lager brewing yeasts is related to lager brewing yeast 2-μm DNA. Curr Genet 22, 357–361 (1992). https://doi.org/10.1007/BF00352436
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DOI: https://doi.org/10.1007/BF00352436