Summary
We have determined the nucleotide sequence of a 5159 base-pair (bp) region of the Chlamydomonas reinhardtii plastome containing three photoelectron transport genes, psbF, psbL and petG, and an unusual open reading frame, ORF712. The photosynthetic genes have an unprecedented arrangement. psbF and psbL are located in close proximity to petG, and are not grouped with two other genes of the cytochrome b559 locus, psbE and ORF42. ORF712, located adjacent to psbL, has homology at its 5′-and 3′-ends to the ribosomal protein rps3 gene, but contains, a central 437 residue domain that lacks similarity to any other known sequence. These sequences add to the growing body of evidence that the chloroplast genome of C. reinhardtii has a significantly different gene arrangement to its counterpart in plants. The structure of ORF712 also provides another example of a phenomenon we have discovered with C. reinhardtii RNA polymerase genes (Fong and Surzycki 1992); namely, that the algal plastome contains chimeric genes in which reading frames with homology to known genes are juxtaposed in-frame with long coding regions of unknown identity.
References
Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB, Sanford JC (1988) Science 240:1534–1538
Cantrell A, Bryant DA (1988) Photosyn Res 16:65–81
Cushman JC, Christopher DA, Little MC, Hallick RB, Price CA (1988) Curr Genet 13:173–180
Fong SE, Surzycki SJ (1992) Curr Genet 21:485–497
Haley J, Bogorad L (1989) Proc Natl Acad Sci USA 86:1534–1538
Hallick RB (1989) Plant Mol Biol Rep 7:266–275
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun C-R, Meng B-Y, Li Y-Q, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194
Ikeuchi M, Takio K, Inoue Y (1989) FEBS Lett 242:263–269
Mayfield SP, Kindle KL (1990) Proc Natl Acad Sci USA 87:2087–2091
McLaughlin WE, Larrinua IM (1987) Nucleic Acids Res 15:4689
Michel F, Dujon B (1983) EMBO J 2:33–38
Ohyama K, Fukuzama H, Kohchi T, Shirai H, Sano T, Sano H, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1988) J Mol Biol 203:281–298
Palmer JD (1991) Plastid chromosomes: Structure and Evolution. In: Bogorad L, Vasil IK (eds) Cell Culturer and Somatic Cell Genetics of Plants, vol 7A Academic Press, New York London pp 5–53
Robertson D, Boynton JE, Gillham NW (1990) Mol Gen Genet 221:155–163
Rochaix J-D (1987) FEMS Microbiol Rev 46:13–34
Shinozaki H, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Stirewalt VL, Bryant DA (1989) Nucleic Acids Res 17:10095
Vitry C de, Diner BA, Popot J-L (1991) J Biol Chem 266:16614–16621
Webber AN, Packman L, Chapman DJ, Barber J, Gray JC (1989) FEBS Lett 242:259–272
Zurawski G, Zurawski SM (1985) Nucleic Acids Res 13:4521–4526
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Communicated by C.W. Birky, Jr.
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Fong, S.E., Surzycki, S.J. Organization and structure of plastome psbF, psbL, petG and ORF712 genes in Chlamydomonas reinhardtii . Curr Genet 21, 527–530 (1992). https://doi.org/10.1007/BF00351664
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DOI: https://doi.org/10.1007/BF00351664