Abstract
The Q4 class I gene has been shown to participate in gene conversion events within the mouse major histocompatibility complex. Its complete genomic nucleotide sequence has been determined. The 5′ half of Q4 resembles H-2 genes more strongly than other Q genes. Its 3′ end, in contrast, is Q-like and contains a translational stop signal in exon 5 which predicts a polypeptide with an incomplete membrane spanning segment. The presence of two inverted B1 repeats suggests that part of the Q4 gene may be mobile within the genome. Gene transfer experiments have shown that the Q4 gene encodes a β 2-microglobulin associated polypeptide of Mr 41 000. A similar protein was found in activated mouse spleen cells. The Q4 polypeptide was found to be secreted both by spleen cells and by transfected fibroblasts and was not detectable on the cell surface. Antibody binding and two-dimensional gel electrophoresis indicate that the Q4 molecule is identical to a mouse class I polypeptide, Qb-1, which has been previously described.
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Arnold, B., Burgert, H. G., Archibald, A. L., and Kvist, S.: Complete nucleotide sequence of the murine H-2Kk kgene. Comparison of three H-2K locus alleles. Nucleic Acids Res. 12: 9472–9487, 1984
Devlin, J. J., Weiss, E. H., Paulson, M., and Flavell, R. A.: Duplicated gene pairs and alleles of class I genes in the Qa-2 region of the murine Mhc: a comparison. EMBO J. 4: 3203–3207, 1985
Dobberstein, B., Garoff, H., Warren, G., and Robinson, P. J.: Cell free translation and membrane insertion of the H-2Dd histocompatibility antigen and β 2 microglobulin. Cell 17: 759–769, 1979
Elder, J. H. and Alexander, S.: Endo-β-N-acetylglucosaminidase F: Endoglycosidase from flavobacterium meningosepticum that cleaves both high mannose and complex glycoproteins. Proc. Natl. Acad. Sci. U.S.A. 79: 4540–4554, 1982
Flaherty, L.: Tla region antigens. In M. E. Dorf (ed.): The Role of the Major Histocompatibility Complex in Immunology, pp. 33–57, Garland STPM Press, New York, 1981
Fujiki, Y., Hubbard, A. L., Fowler, S., and Lazarow, P. B.: Isolation of intracellular membranes by means of sodium carbonate treatment. Application to endoplasmic reticulum. J. Cell Biol. 93: 97–102, 1982
Geliebter, J., Zeff, R. A., Schulze, D. H., Pease, L. R., Weiss, E. H., Mellor, A. L., and Nathenson, S. G.: Interaction between K band Q4 gene sequences generates the Kb bm1mutation. Mol. Cell. Biol. 6: 645–652, 1986
Harris, R. A., Hogarth, P. M., Penington, D. G., and McKenzie, I. F. C.: Qa antigens and their differential distribution on lymphoid, myeloid and stem cells. J. Immunogenet. 11: 265–281, 1984
Jaulin, C., Perrin, A., Abastado, J. P., Dumas, B., Papamatheakis, J., and Kourilsky, P.: Polymorphism in mouse and human class I H-2 and HLA genes is not the result of random independent point mutations. Immunogenetics 22: 453–470, 1985
Kalb, V. F., Glasser, S., King, D., and Lingrel, J. B.: A cluster of repetitive elements within a 700 base pair region of the mouse genome. Nucleic Acid Res. 11: 2177–2184, 1983
Kimura, A., Israel, A., Le Bail, O., and Kourilsky, P.: Detailed analysis of the mouse H-2K bpromoter: enhancer-like sequences and their role in the regulation of class I gene expression. Cell 44: 261–272, 1986
Lew, A. M., Maloy, W. L., and Coligan, J. E.: Characteristics of the expression of the murine soluble class I molecule, Q10. J. Immunol. 136: 254–258, 1986
Maxam, A. M. and Gilbert, W.: Sequencing end-labeled DNA with base-specific chemical cleavages. Proc. Natl. Acad. Sci. U.S.A. 74: 560–564, 1977
Mellor, A. L., Weiss, E. H., Kress, M., Jay, G., and Flavell, R. A.: A non-polymorphic class I gene in the murine MHC. Cell 36: 139–144, 1984
Mellor, A. L., Antoniou, J., and Robinson, P. J.: Structure and expression of genes encoding murine Qa-2 class I antigens. Proc. Natl. Acad. Sci. U.S.A. 82: 5920–5924, 1985
Michaelson, J., Flaherty, L., Bushkin, Y., and Yudkowitz, H.: Further biochemical data on Qa-2. Immunogenetics 14: 129–140, 1981
Nathenson, S. G., Geliebter, J., Pfaffenbach, G. M., and Zeff, R. A.: Murine major histocompatibility complex class I mutants: molecular analysis and structure-function implications. Annu. Rev. Immunol. 4: 471–502, 1986
Ploegh, H. L., Orr, H. T., and Strominger, J.: Major histocompatibility antigens: the human (HLA A, B, C) and murine (H-2K, H-2D) class I molecules. Cell 24: 287–299, 1981
Reyes, A. A., Schöld, M., and Wallace, B.: The complete amino acid sequence of the murine transplantation antigen H-2Db as deduced by molecular cloning. Immunogenetics 6: 1–9, 1982
Robinson, P. J.: Qb-1, a new class I polypeptide encoded by the Qa region of the mouse H-2 complex. Immunogenetics 22: 285–289, 1985
Robinson, P. J.: Two different pathways for the secretion of Qa region associated class I antigens by mouse lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 84: 527–531, 1987
Robinson, P. J. and Schirrmacher, V.: Differences in the expression of histocompatibility antigens on mouse lymphocytes and tumour cells: immunochemical studies. Eur. J. Immunol. 9: 61–66, 1979
Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. G. H., and Roe, B. A.: Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J. Mol. Biol. 143: 161–178, 1980
Soloski, M. J., Vernachio, J., Einhorn, G., and Lattimore, A.: Qa gene expression: biosynthesis and secretion of Qa-2 molecules in activated T cells. Proc. Natl. Acad. Sci. U.S.A. 83: 2949–2953, 1986
Steinmetz, M., Moore, K. W., Frelinger, J. G., Sher, B., Shen, F. W., Boyse, E. A., and Hood, L.: A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell 25: 683–692, 1981
Taylor Sher, B., Nairn, R., Coligan, J. E., and Hood, L. E.: DNA sequence of the mouse H-2D dtransplantation antigen gene. Proc. Natl. Acad. Sci. U.S.A. 82: 1175–1179, 1985
Weiss, E. H., Golden, L., Zakut, R., Mellor, A. L., Fahrner, K., Kvist, S., and Flavell, R. A.: The DNA sequence of the H-2K bgene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibility antigens. EMBO J. 2: 453–462, 1983
Weiss, E. H., Golden, L., Fahrner, K., Mellor, A. L., Devlin, J. J., Bullman, H., Tiddens, H., Bud, H., and Flavell, R. A.: Organisation and evolution of the class I gene family in the major histocompatibility complex of the C57BL/6 mouse. Nature 310: 650–655, 1984
Wigler, M. S., Silverstein, L. S., Lee, A., Pellicer, Y., Cheng, T., and Axel, R.: Transfer of purified herpes virus thymidine kinase gene into cultured mouse cells.Cell 11: 223–232, 1977
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Robinson, P.J., Bevec, D., Mellor, A.L. et al. Sequence of the mouse Q4 class I gene and characterization of the gene product. Immunogenetics 27, 79–86 (1988). https://doi.org/10.1007/BF00351079
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DOI: https://doi.org/10.1007/BF00351079