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Two genes encode 7SL RNAs in the yeast Yarrowia lipolytica

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Summary

We have identified an abundant cytoplasmic 7S RNA in crude extracts of the yeast Yarrowia lipolytica. A cDNA probe was prepared from this RNA and used to screen a genomic library. The DNA sequence of a positive clone was determined and the end positions of the 7S RNA gene established by comparison with the sequence of the extremities of 7S RNA. This gene, designated SCR2, encodes a 270-nucleotide RNA that can be folded into a secondary structure similar to that of 7SL RNAs. This RNA is 94.4% homologous to a previously identified 7S RNA from this yeast, but is encoded by a separate gene with highly divergent flanking sequences.

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Author notes

  1. V. Ribes & D. Tollervey

    Present address: European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, FRG

Authors and Affiliations

  1. Laboratoire de génétique des microorganismes, Institut National Agronomique, F-78850, Thiverval Grignon, France

    F. He, J. M. Beckerich & C. M. Gaillardin

  2. Institut Pasteur, 28 rue du Docteur Roux, F-75015, Paris, France

    V. Ribes & D. Tollervey

Authors
  1. F. He
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  2. J. M. Beckerich
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  3. V. Ribes
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  4. D. Tollervey
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  5. C. M. Gaillardin
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He, F., Beckerich, J.M., Ribes, V. et al. Two genes encode 7SL RNAs in the yeast Yarrowia lipolytica . Curr Genet 16, 347–350 (1989). https://doi.org/10.1007/BF00340713

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  • DOI: https://doi.org/10.1007/BF00340713

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