Summary
We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Using these mutant mini-F plasmids, we found that F3 protein is essential for the replication of mini-F from origin I, but not from origin II.
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Communicated by M. Takanami
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Tanimoto, K., Iino, T. An essential gene for replication of the mini-F plasmid from origin I. Mol Gen Genet 196, 59–63 (1984). https://doi.org/10.1007/BF00334092
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DOI: https://doi.org/10.1007/BF00334092