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Delayed observation of laser-induced fluorescence for imaging of tumors

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Abstract

We report on a technique to improve fluorescence images of superficially growing tumors marked with photosensitizers. Exploiting the longer fluorescence decay times of porphyrin-based photosensitizers compared to average decay times of tissue autofluorescence, delayed detection of laser-induced fluorescence allows to suppress the autofluorescence background. The feasibility of delayed fluorescence imaging of tumors has been demonstrated in-vitro. It follows from an analysis of delayed fluorescence spectra that autofluorescence background falling into the photosensitizer fluorescence band can be reduced by up to one order of magnitude.

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References

  1. 1.A.E. Profio: IEEE J. QE-20, 1502 (1984)

    Google Scholar 

  2. A.E. Profio: Laser Surgery: Characterization and Therapeutics, SPIE Vol. 907 (The International Society for Optical Engineering, Bellingham 1988) pp. 150–6

    Google Scholar 

  3. S. Andersson-Engels, J. Johansson, U. Stenram, K. Svanberg, S. Svanberg: IEEE J. QE-26, 2207 (1990)

    Google Scholar 

  4. S. Andersson-Engels, J. Johansson, K. Svanberg, S. Svanberg: Photochem. Photobiol. 53, 807 (1991)

    Google Scholar 

  5. R.R. Alfano, G.C. Tang, A. Pradhan, W. Lam, D.S.J. Choy, E. Opher: IEEE J. QE-23, 1806 (1987)

    Google Scholar 

  6. M. Sartori, R. Sauerbrey, S. Kubodera, F.K. Tittel, R. Roberts, P.D. Henry: IEEE J. QE-23, 1794 (1987)

    Google Scholar 

  7. S. Lam, J. Hung, C. MacAulay, B. Jaggi, B. Palcic: Annual Int'l Conf. IEEE Engineering in Medicine and Biology Soc. 12, 1142 (1990)

    Google Scholar 

  8. R. Richards-Kortum, R.P. Rava, M. Fitzmaurice, L.L. Tong, N.B. Ratliff, J.R. Kramer, M.S. Feld: IEEE Trans. BE-36, 1222 (1989)

    Google Scholar 

  9. R. Richards-Kortum, R.P. Rava, R.E. Petras, M. Fitzmaurice, M. Sivak, M.S. Feld: Photochem. Photobiol. 53, 777 (1991)

    Google Scholar 

  10. G. Renault, M. Muffat-Joly, J. Polianski, R.I. Hardy, R. Nottin, M. Weiss, J.-L. Boutineau, J.-P. Binet, J.-J. Pocidalo: Optical Fibers in Medicine II, SPIE Vol. 713 (The International Society for Optical Engineering, Bellingham 1988) pp. 158–165

    Google Scholar 

  11. J. Beuthan, Ch. Zur, F. Fink, G. Müller: Laser Med. Surg. 6, 72 (1990)

    Google Scholar 

  12. G. Morstyn, A.H. Kaye: Phototherapy of Cancer (Harwood, Chur 1990)

    Google Scholar 

  13. R.D. Barabash, J.S. McCaughan, A.S. Kolobanov, G. Wolken, V.E. Normansky, J.A. Walker: IEEE J. QE-26, 2226 (1990)

    Google Scholar 

  14. E. Unsöld, R. Baumgartner, W. Beyer, D. Jocham, H. Stepp: Lasers Med. Sci. 5, 207 (1990)

    Google Scholar 

  15. M.C. Luna, C.J. Gomer: Cancer Res. 51, 4243 (1991)

    Google Scholar 

  16. T. Okunaka, H. Kato, C. Konaka, N. Kawate, A. Bonaminio, H. Yamamoto, N. Ikeda, M. Tolentino, M.L. Eckhauser, Y. Hayata: Cancer 68, 253 (1991)

    Google Scholar 

  17. M. Kohl, J. Neukammer, U. Sukowski, H. Rinneberg, E.A. Friedrich, H.-J. Sinn, D. Wöhrle, P. Schlag, G. Graschew, W. Maier-Borst: Proc. Int'l Conf. Optics in Life Sciences 1990, ed. by G. von Bally, Springer Ser. Opt. Sci. (Springer, Berlin, Heidelberg) in press

    Google Scholar 

  18. M. Kohl, J. Neukammer, U. Sukowski, H. Rinneberg, H.-J. Sinn, E.A. Friedrich, G. Graschew, P. Schlag, D. Wöhrle: In Laser in der Medizin, ed. by W. Waidelich, R. Waidelich, A. Hofstetter (Springer, Berlin Heidelberg 1992) pp. 312–316

    Google Scholar 

  19. M. Kohl, J. Neukammer, U. Sukowski, H. Rinneberg, H.-J. Sinn, E.A. Friedrich, G. Graschew, P. Schlag, D. Wöhrle: Future Trends in Biomedical Applications of Lasers, SPIE Vol. 1525 (The International Society for Optical Engineering, Bellingham 1991) pp. 26–34

    Google Scholar 

  20. R. Cubeddu, P. Taroni, G. Valentini: J. Photochem. Photobiol. B: Biol. 12, 107 (1992)

    Google Scholar 

  21. R. Baumgartner, H. Fisslinger, D. Jocham, H. Lenz, L. Ruprecht, H. Stepp, E. Unsöld: Photochem. Photobiol. 46, 759 (1987)

    Google Scholar 

  22. Ph. Monnier, M. Savary, Ch. Fontolliet, G. Wagnières, A. Châtelain, P. Cornaz, Ch. Depeursinge, H. van den Bergh: Lasers Med. Sci. 5, 149 (1990)

    Google Scholar 

  23. G. Wagnières, D. Braichotte, A. Châtelain, Ch. Depeursinge, Ph. Monnier, M. Savary, Ch. Fontolliet, J.-M. Calmes, J.-C. Givel, G. Chapuis, S. Folli, A. Pélegrin, F. Buchegger, J.-P. Mach, H. van den Bergh: In Future Trends in Biomedical Applications of Lasers, SPIE Vol. 1525 (The International Society for Optical Engineering, Bellingham 1991) pp. 219–236

    Google Scholar 

  24. I.B. Berlman: Handbook of Fluorescence Spectra of Aromatic Molecules, 2nd edn. (Academic, New York 1971)

    Google Scholar 

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Authors and Affiliations

  1. Physikalisch-Technische Bundesanstalt, Berlin-Charlottenburg, Abbestrasse 2-12, W-1000, Berlin 10, Germany

    M. Kohl, J. Neukammer, U. Sukowski & H. Rinneberg

  2. Institut für Organische und Makromolekulare Chemie, Universität Bremen, Leobener Strasse NW 2, W-2800, Bremen 33, Germany

    D. Wöhrle

  3. Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, W-6900, Heidelberg, Germany

    H.-J. Sinn & E. A. Friedrich

Authors
  1. M. Kohl
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  2. J. Neukammer
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  3. U. Sukowski
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  4. H. Rinneberg
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  5. D. Wöhrle
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  6. H.-J. Sinn
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  7. E. A. Friedrich
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Kohl, M., Neukammer, J., Sukowski, U. et al. Delayed observation of laser-induced fluorescence for imaging of tumors. Appl. Phys. B 56, 131–138 (1993). https://doi.org/10.1007/BF00332192

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  • DOI: https://doi.org/10.1007/BF00332192

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