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A new mutator strain of Bacillus subtilis

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Summary

Bacillus subtilis strain SB1207, widely used in our laboratory, was found to be highly temperature-sensitive and to exhibit a strong SOS-independent mutator phenotype at elevated temperatures. Both chromosomal and plasmid-borne genes were affected by the mutator. Lethality and mutator phenotype could not be attributed to a replication shut off or to thymine starvation. Due to the high frequency of base misincorporation, the mutator phenotype probably results from an editing defect rather than from a post-replication defect (mismatch repair).

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References

  • Alonso JC, Trautner TA (1985) A gene controlling segregation of the Bacillus subtilis plasmid pC194. Mol Gen Genet 198:427–431

    Google Scholar 

  • Alonso JC, Viret J-F, Tailor RH (1987) Plasmid maintenance in Bacillus subtilis recombination deficient mutants. Mol Gen Genet 208:349–352

    Google Scholar 

  • Biswal N, Kleinschmidt AK, Spatz HC, Trautner TA (1967) Physical properties of the DNA of bacteriophage SP50. Mol Gen Genet 100:39–55

    Google Scholar 

  • Bresler SE, Mosevitsky MI, Vyacheslavov LG (1973) Mutations as possible replication errors in bacteria growing under conditions of thymine deficiency. Mutat Res 19:281–293

    Google Scholar 

  • Burton K (1956) A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323

    Google Scholar 

  • Clark AJ, Volkert MR, Margossian LJ, Nagaishi H (1982) Effects of a recA operator mutation on mutant phenotypes conferred by lexA and recF mutations. Mutat Res 106:11–26

    Google Scholar 

  • Cox EC (1976) Bacterial mutator genes and the control of spontatneous mutation. Annu Rev Genet 10:135–156

    Google Scholar 

  • Dedonder RA, Lepesant J-A, Lepesant-Kejzlarova J, Billault A, Steinmetz M, Kunst F (1977) Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol 33:989–993

    Google Scholar 

  • de Vos WM, de Vries SC, Venema G (1983) Cloning and expression of the Escherichia coli recA gene in Bacillus subtilis. Gene 25:301–308

    Google Scholar 

  • Fridman BM, Yasbin RE (1983) The genetics and specificity of the constitutive excision repair system of Bacillus subtilis. Mol Gen Genet 190:481–486

    Google Scholar 

  • Gass KB, Cozzarelli NR (1973) Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases. J Biol Chem 248:7688–7700

    Google Scholar 

  • Georgopoulos CP (1969) Suppressor system in Bacillus subtilis 168. J Bacteriol 97:1397–1402

    Google Scholar 

  • Gross JD, Karamata D, Hempstead PG (1968) Temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Cold Spring Harbor Symp Quant Biol 33:307–312

    Google Scholar 

  • Hara H, Yoshikawa H (1973) Asymmetrical bidirectional replication of Bacillus subtilis chromosome. Nature (New Biol) 244:200–203

    Google Scholar 

  • Harford N (1974) Genetic analysis of rec mutants of Bacillus subtilis: Evidence for at least six linkage groups. Mol Gen Genet 129:269–274

    Google Scholar 

  • Iglesias A, Trautner TA (1983) Plasmid transformation in Bacillus subtilis: Symmetry of gene conversion in transformation with a hybrid plasmid containing chromosomal DNA. Mol Gen Genet 189:73–76

    Google Scholar 

  • Karamata D, Gross JD (1970) Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA replication. Mol Gen Genet 108:277–287

    Google Scholar 

  • Kunz BA (1982) Genetic effects of deoxyribonucletide pool imbalances. Environ Mutagen 4:695–725

    Google Scholar 

  • Loeb LA, Kunkel TA (1982) Fidelity of DNA synthesis. Annu Rev Biochem 52:429–457

    Google Scholar 

  • Love E, D'Ambrosio J, Brown NC, Dubnau D (1976) Mapping of the gene specifying DNA polymerase III of Bacillus subtilis. Mol Gen Genet 144:313–321

    Google Scholar 

  • Mazza G, Galizzi A (1978) The genetics of DNA replication, reparir and recombination in Bacillus subtilis. Microbiologica 1:111–135

    Google Scholar 

  • Piggot PJ, Hoch JA (1985) Revised genetic linkage map of Bacillus subtilis. Microbiol Rev 49:158–179

    Google Scholar 

  • Sadaie Y, Narui K (1976) Repair deficiency, mutator activity, and thermal prophage inducibility in dna-8132 strains of Bacillus subtilis. J Bacteriol 126:1037–1041

    Google Scholar 

  • Sargentini NJ, Smith KC (1985) Spontaneous mutagenesis: the roles of DNA repair, replication, and recombination. Mutat Res 154:1–27

    Google Scholar 

  • Spizizen J (1958) Transformation of biochemically deficient strains of B. subtilis by deoxyribonucleate. Proc Natl Acad Sci USA 44:1072–1078

    Google Scholar 

  • Viret J-F, Rogers HJ, Karamata D (1985) Morphological and cell wall alteration in thermosensitive dna mutants of B. subtilis. Ann Microbiol (Paris) 136A:119–129

    Google Scholar 

  • Yasbin RE, Fields PI, Andersen BJ (1980) Properties of Bacillus subtilis 168 derivatives freed of their natural prophages. Gene 12:155–159

    Google Scholar 

  • Zahler SA, Korman RZ, Rosenthal R, Hemphill HE (1977) Bacillus subtilis bacteriophage SPβ: Localization of the prophage attachment site, and specialized transduction. J Bacteriol 129:556–558

    Google Scholar 

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Communicated by W. Goebel

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Viret, JF., Alonso, J.C. A new mutator strain of Bacillus subtilis . Mole Gen Genet 208, 353–356 (1987). https://doi.org/10.1007/BF00330465

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  • DOI: https://doi.org/10.1007/BF00330465

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