Summary
The distribution of neural crest derived cells (NC) in the heart of quail-chick chimeric embryos was analyzed three-dimensionally after computer reconstruction. During the division of the truncus arteriosus into the aorta and the pulmonary trunk, ventral and dorsal columns of NC-derived cells were found in the truncal swellings. These columns were elongations from the aorticopulmonary (AP) septum. The dorsal column extended more proximally than did the ventral column. Around hatching, NC-derived cells located between the proximal aorta and the pulmonary trunk, differentiated into cartilage and connective tissue. They formed a part of the cardiac skeleton. A small number of NC-derived cells were scattered in the cusps of the arterial valves. Cells derived from the right NC were located around the aorta and the right arch arteries but not around the distal pulmonary trunk and the left arch arteries. At the proximal level, cells derived from the rigth NC were located in both the dorsal and ventral columns. These results suggest that the AP septum is mainly formed by NC-derived cells, right and left NC cells migrating into assigned areas in the heart. Location of two columns of NC-derived cells may support a translocation hypothesis for the AP septum during truncal division.
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Akimoto N, Satow Y, Lee JY, Sumida H, Nakamura H, Okamoto N (1986) Neural crest cells and division of conotruncus in birds. (In Japanese with an English abstract) Proc Hiroshima Univ RINMB 27:211–233
Feulgen R, Rossenbeck H (1924) Mikroskopisch-chemischer Nachweis einer Nucleinsäure von Typus der Thymonucleinsäure und die darauf beruhende elektive Färbung von Zellkernen in mikroscopischen Präperaten. Hoppe-Seyler's Z Physiol Chem 135:203–248
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Kirby ML (1987) Cardiac morphogenesis — Recent research advances. Pediatr Res 21:219–224
Kirby ML, Gale TF, Stewart DE (1983) Neural crest cells contribute to normal aorticopulmonary septation. Science 220:1059–1061
Kirby ML, Turnage KL III, Hays BM (1985) Characterization of conotruncal malformations following ablation of “cardiac” neural crest. Anat Rec 213:87–93
Laane H-M (1978) The septation of the arterial pole of the heart in the chick embryo. II. Development of the truncus arteriosus of the heart of chick embryos from 4 to 5 days of incubation. Acta Morphol Neerl-Scand 16:29–53
Laane H-M (1979) The septation of the arterial pole of the heart in the chick embryo. III. Development of the truncus arteriosus of the heart of chick embryos from 5 2/1 to 7 days of incubation. Acta Morphol Neerl-Scand 17:1–20
Le Lievre CS, Le Douarin NM (1975) Mesenchymal derivatives of the neural crest: Analysis of chimaeric quail and chick embryos. J Embryol Exp Morphol 34:125–154
Los JA (1978) Cardiac septation and development of the aorta, pulmonary trunk, and pulmonary veins: Previous work in the light of recent observations. Birth Defects 14(7):109–138
Okamoto N (1980) Morphologic aspects of the developing human heart. In: Congenital anomalies of the heart. Igaku-Shoin, Tokyo, pp 22–58
Okamoto N, Akimoto N, Satow Y, Hidaka N, Miyabara S (1981) Role of cell death in conal ridges of developing human heart. In: Pexieder T (ed) Perspectives in cardiovascular research, vol 5. Raven Press, New York, pp 127–137
Stiefel K (1926) Das Herz des melanotischen Seidenhuhns. Anat Anz 61:177–210
Sumida H, Nakamura H, Akimoto N, Okamoto N, Satow Y (1987) Desmin distribution in the cardiac outflow tract of the chick embryo during aortico-pulmonary septation. Arch Histol Jpn 50:525–531
Sumida H, Ashcraft RA Jr, Thompson RP (1989) Cytoplasmic stress fibers in the developing heart. Anat Rec 223:82–89
Thompson RP, Wong Y-M, Fitzharris TP (1983) A computer graphic study of cardiac truncal septation. Anat Rec 206:207–214
Thompson RP, Wong Y-M, Fitzharris TP (1984) Patterns of tensil stress in the developing cardiac truncus. In: Nora JJ, Takao A (eds) Congenital heart disease: Causes and processes. Futura, Mount Kisco, pp 387–400
Van Mierop LSH (1969) Embryology. In: Netter FH, Yonkman FF (eds), The Ciba collection of medical illustration, vol 5. Ciba Foundation, New York, pp 119–123
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Sumida, H., Akimoto, N. & Nakamura, H. Distribution of the neural crest cells in the heart of birds: a three dimensional analysis. Anat Embryol 180, 29–35 (1989). https://doi.org/10.1007/BF00321897
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DOI: https://doi.org/10.1007/BF00321897