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Assessment of inner filter effect corrections in fluorimetry

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  • General Chemistry
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Summary

The inner filter effect (IFE) in fluorescence spectroscopy is not easily distinguished from dynamic and static quenching phenomena, since IFE rarely occurs without quenching. IFE corrections may be subject to under-or over-compensation effects that are difficult to assess accurately. To evaluate existing IFE correction procedures, it is proposed that the linearity of resulting Stern-Volmer plots and the relative change of their slopes with temperature be adopted as criteria. Three correction methods are assayed in this manner, and the equation described by Gauthier et al. is found to produce the best results.

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Authors and Affiliations

  1. Division of Soil Science, University of Idaho, 83843, Moscow, ID, USA

    M. M. Puchalski & M. J. Morra

  2. Department of Chemistry, University of Idaho, 83843, Moscow, ID, USA

    R. von Wandruszka

Authors
  1. M. M. Puchalski
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  2. M. J. Morra
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  3. R. von Wandruszka
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Puchalski, M.M., Morra, M.J. & von Wandruszka, R. Assessment of inner filter effect corrections in fluorimetry. Fresenius J Anal Chem 340, 341–344 (1991). https://doi.org/10.1007/BF00321578

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  • DOI: https://doi.org/10.1007/BF00321578

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