Abstract
The relationship between the expression of adult T-cell leukemia-derived factor, human thioredoxin (ADF/TRX), and rejection in transplanted canine lungs was investigated in this study. Of a total 27 adult mongrel dogs, 24 underwent allotransplantation of the left lung with no immunosuppressant and the other three underwent autotransplantation of the left lung. Of the allotransplanted dogs, five were killed on postoperative day (POD) 1, five on POD 2, seven on POD 3, and seven on POD 5, while all three autotransplanted dogs were killed on POD 5. Histological examination was performed on the 24 allotransplanted left lungs (group A), 12 autologous right lungs (group B), and bilateral lungs of the three autotransplanted dogs (group C). The lung tissue was stained with anti-ADF antibody, and the high-ADF-producing cells (ADFh cells) in a randomly chosen field were counted as an index of ADF expression. As the signs of rejection in the group A lungs became more severe with time, the ADFh cells increased in number: 1.68±1.15, 6.08±3.44, 14.03±6.09, and 47.74±18.89, on PODs 1, 2, 3, and 5, respectively. However, the number of ADFh cells in the group B and group C lungs did not become significantly different from that on POD 1 in group A. These results suggested that ADF/TRX expression may be useful for the early diagnosis of rejection of transplanted lungs.
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Okada M, Maeda M, Tagaya Y, Taniguchi Y, Teshigawara K, Yoshiki T, Diamanstein T, Smith KA, Uchiyama T, Honjo T, Yodoi J (1985) TCGF (IL-2) receptor inducing factor(s). II. Possible role of ATL-derived factor (ADF) on constitutive IL-2 receptor expression of HTLV-1 (+) T cell lines. J Immunol 135:3995–4003
Teshigawara K, Maeda M, Nishino K, Uchiyama T, Tsudo M, Wano Y, Yodoi J (1985) Adult T leukemia cells produce a lymphokine that augments interleukin 2 receptor expression. J Mol Immunol 2:17–26
Tagaya, Y, Taniguchi Y, Nakamura M, Okada M, Suzuki N, Kanamori N, Nikaido T, Honjo T, Yodoi J (1987) Transcription of IL-2 receptor gene is stimulated by ATL-derived factor produced by HTLV-1 (+) T cell lines. Immunol Lett 15:221–228
Holmgren A (1985) Thioredoxin. Ann Rev Biochem 54:237–271
Wollman EE, Auriol L, Rimsky L, Shaw A, Jaequot JP, Wingfield P, Graber P, Dessarps F, Robin P, Galibert F, Bertoglio J, Fradelizi D (1988) Cloning and expression of cDNA for human thioredoxin. J Biol Chem 263:15506–15512
Laurent TC, Moore EC, Richard P (1986) Enzymatic synthesis of deoxyribonucleotides. Isolation and characterization of thioredoxin, the hydrogen donor from Escherichia coli. J Biol Chem 239:3436–3444
Tagaya Y, Maeda Y, Mitsui A, Kondo N, Matsui N, Hamuro J, Brown N, Arai K, Yokota T, Wakasugi T, Yodoi J (1989) ATL-derived factor (ADF), an IL-2 receptor/Tac inducer homologous to thioredoxin; possible involvement of dithiol-reduction in the IL-2 receptor induction. EMBO J 8:757–764
Mitsui A, Hirkawa T, Yodoi J (1992) Reactive oxygen-reducing and protein refolding activities of adult T-cell leukemia-derived factor/human thioredoxin. Biochem Biophys Res Commun 186:1220–1226
Schallreuter KU, Wood JM (1986) The role of thioredoxin reductase in the reduction of free radicals at the surface of the epidermis. Biochem Biophys Res Commun 136:630–637
Spector A, Yan GZ, Huang RRC, McDermott MJ, Gascoine PRC, Pigiet V (1988) The effect of H2O2 upon thioredoxin-enriched lens epithelial cells. J Biol Chem 263:4984–4990
Pigiet VP, Shuster BJ (1986) Thioredoxin-catalyzed refolding of disulfide-containing proteins. Proc Natl Acad Sci USA 83:7643–7647
Veith FJ, Richards K (1970) Improved technique for canine lung transplantation. Ann Surg 171:553–558
Hasegawa S, Wada H, Yokomise H, Hirai T, Fukuse T, Muro K, Takahasi Y, Inui K, Aoki M, Hitomi S (1992) Combination use with suboptimal dosage of FK506 and Cyclosporine A in canine lung transplantation. J Thorac Cardiovasc Surg 104:1340–1348
Hirai T, Wada H, Hitomi S, Hasegawa S, Inui K, Yokomise H, Yamazaki F, Aoki M (1992) The immunosuppressive effect of FK 506 on canine lung transplantation. J Thorac Cardiovasc Surg 103:1127–1135
Yamazaki F, Wada H, Aoki M, Inui K, Hitomi S (1989) An evaluation of the tolerance of the autotransplanted canine lung against warm ischemia. Jpn J Surg 19:326–333
Yokomise H, Wada H, Inui K, Yamazaki H, Lee M, Aoki M, Hitomi S (1989) Application of laser doppler velocimetry to lung transplantation. Transplantation 48:550–554
Tagaya Y, Okada M, Sugie K, Kasahara T, Kondo K, Hamuro J, Matsushima K, Dinarello CA, Yodoi J (1988) IL-2 receptor/Tac(p55) inducing factor: Purification and characterization of ATL-derived factor (ADF). J Immunol 140:2613–2620
Prop J, Wildevuur CRH, Nieuwenhuis P (1985) Lung allograft rejection in the rat. (2) Specific immunological properties of lung grafts. Transplantation 40:126–131
Kahn HJ, Marks A, Thom H, Baumal R (1983) Role of antibody to S-100 protein in diagnostic pathology. Am J Clin Pathol 79:341–347
Tanaka Y (1986) Immunocytochemical study of human lymphoid tissues with monoclonal antibodies against S-100 protein subunits. Virchow Arch A 410:125–132
Masutani H, Naito M, Takahashi K, Hattori T, Koito A, Takatsuki K, Go T, Nakamura H, Fujii S, Yoshioka Y, Okuma M, Yodoi J (to be published) Dysregulation of Adult T-Cell Leukemia-Derived Factor (ADF)/Thioredoxin in HLV Infection: Loss of ADF High-Producer Cells in Lymphoid Tissues of AIDS Patients. AIDS Res Hum Retroviruses
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Muro, K., Go, T., Hirata, T. et al. Expression of the adult T-cell leukemia-derived factor, human thioredoxin, in the allotransplanted canine lung. Surg Today 25, 626–632 (1995). https://doi.org/10.1007/BF00311437
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DOI: https://doi.org/10.1007/BF00311437