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A novel synthetic vitamin D analogue, 2β-(3-hydroxypropoxy)1α, 25-dihydroxyvitamin D3 (ED-71), increases bone mass by stimulating the bone formation in normal and ovariectomized rats

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Abstract

We performed dosing experiments to evaluate the bone mass increasing action of a novel, synthetic vitamin D derivative, 2β-(3-hydroxypropoxy)-1α, 25(OH)2D3 (ED-71), in normal and estrogen-deficient rats. The first experiment consisted of 31 Sprague-Dawley rats, 28 weeks of age. The second experiment consisted of 44 animals who were ovariectomized (OVX) or sham operated at the age of 12 weeks. ED-71 was given twice a week for the duration of 12 weeks. At the end of the experiments, serum chemistries were examined and lumbar vertebrae were assessed histomorphometrically. Serum alkaline-phosphatase levels tended to decrease by ED-71 administration in the first experiment and their elevated values after ovariectomy were also depressed by ED-71 in the second experiment. Serum osteocalcin levels, however, increased by the agent. In the first experiment, cancellous bone volume (BV/TV) increased dose dependently. Bone formation rates (BFR/BS) also increased. In the second experiment, BV/TV significantly decreased by ovariectomy and it increased in ED-71-treated groups, but not in 1α-(OH)D3-treated group. BFR/BS increased by ED-71. Activation frequency did not decreased by ED-71 in either experiment. These data clearly demonstrated that ED-71 administration was capable of increasing the bone mass by stimulating bone formation in normal and estrogen-deficient rats.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, 807, Kitakyushu, Japan

    H. Tsurukami, T. Nakamura & K. Suzuki

  2. Central Research Laboratories, Chugai Pharmaceutical Co. Ltd., 3-41-8 Takada, Toshima-ku, 171, Tokyo, Japan

    K. Sato, Y. Higuchi & Y. Nishii

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  1. H. Tsurukami
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  2. T. Nakamura
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  3. K. Suzuki
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  4. K. Sato
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  5. Y. Higuchi
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  6. Y. Nishii
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Tsurukami, H., Nakamura, T., Suzuki, K. et al. A novel synthetic vitamin D analogue, 2β-(3-hydroxypropoxy)1α, 25-dihydroxyvitamin D3 (ED-71), increases bone mass by stimulating the bone formation in normal and ovariectomized rats. Calcif Tissue Int 54, 142–149 (1994). https://doi.org/10.1007/BF00296065

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  • DOI: https://doi.org/10.1007/BF00296065

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