Summary
Freshly delivered human placentas were exposed to ultrasound for 30 min using a diagnostic linear array unit. Blood was then drawn and cultured in the presence of bromodeoxyuridine, and the frequencies of sister chromatid exchanges (SCE) in the lymphocytes determined. There was no statistically significant difference in SCE frequencies between control and exposed cells; the frequencies of SCEs per cell ranged from 4.50 to 6.02 for control and from 4.66 to 6.10 for exposed cells in five separate experiments. Positive control mitomycin C treated cells were significantly affected, with more than 50 SCEs per cell.
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Au W, Obergonner N, Goldenthal K, Corry P, Willingham V (1982) Sister chromatid exchanges in mouse embryos after exposure to ultrasound in utero. Mutat Res 103:315–320
Barnett SB, Bonin A, Mitchell G, Meher-Honji KM, Baker RSV (1982) An investigation of the mutagenic potential of pulsed ultrasound. Br J Radiol 55:501–504
Barnett SB, Baker RSV, Barnstable S (to be published) Is pulsed ultrasound mutagenic? Proceedings World Fed. Ultrasound Med Biol
Barrass N, Ter Haar G, Casey G (1982) The effect of ultrasound and hyperthermia on sister chromatid exchange and division kinetics of BHK 21 C13/A3 cells. Br J Cancer 45:187–191
Brulfert A, Ciaravino V, Miller MW, Carstensen EL (to be published) Lack of ultrasound effect on in vitro human lymphocyte sister chromatid exchange. Ultrasound Med Biol
Carstensen EL, Parker KJ, Barbee DB (to be published) Temporal peak intensity. J Acoust Soc Am
Ehlinger CA, Katayama PK, Roester MR, Mattingly RF (1981) Diagnostic ultrasound increases sister chromatid exchange; preliminary report. Wis Med J 80:21–22
Haupt M, Martin N, Simpson JL, Iqbal M, Elias S, Dyer A, Sabbagha RE (1981) Ultrasonic induction of sister chromatid exchanges in human lymphocytes. Hum Genet 39:221–226
Latt SA (1974) Sister chromatid exchanges, indices of human chromosome damage and repair: detection by fluorescence and induction by mitomycin C. Proc Natl Acad Sci USA 71:3162–3166
Liebeskind D, Bases R, Elequin F, Neunott S, Liefer R, Golberg R, Koenigsberg M (1979a) Diagnostic ultrasound: effects on the DNA and growth patterns of animal cells. Radiology 131:177–184
Liebeskind D, Bases R, Mendez F, Elequin F, Koenigsberg M (1979b) Sister chromatid exchanges in human lymphocytes after exposure to diagnostic ultrasound. Science 205:1273–1275
Linnainmaa K, Wolff S (1982) Sister chromatid exchange induced by short-lived monoadducts produced by the bifunctional agents mitomycin C and 8-methoxypsoralen. Environ Mutagen 4:239–247
Lundberg M, Leslie J, Livingston G, Kochenour N, Lee T, Fineman R (1982) Failure to demonstrate an effect of in vivo diagnostic ultrasound on sister chromatid exchange frequency in amniotic fluid cells. Am J Med Genet 11:31–35
Miller MW, Wolff S, Filly R, Cox C, Carstensen EL (1983) Absence of an effect of diagnostic ultrasound on sister chromatid exchange induction in human lymphocytes in vitro. Mutat Res 120:261–268
Morris SM, Palmer CG, Fry FJ, Johnson LK (1978) Effect of ultrasound on human leucocytes. Sister chromatid exchange analysis. Ultrasound Med Biol 4:253–258
N.C.R.P. (1984) Biological effects of ultrasound: mechanisms and clinical applications. Scientific Committee No. 66. National Council for Radiation Protection and Measurements. Bethesda
Perry P, Wolff S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158
Wegner RD, Obe G, Meyenburg M (1980) Has diagnostic ultrasound mutagenic effects? Hum Genet 56:95–98
Wegner RD, Meyenburg M (1982) The effects of diagnostic ultrasonography on the frequencies of sister chromatid exchanges in Chinese hamster cells and human lymphocytes. J Ultrasound Med 1:355–358
Zheng HZ, Mitter NS, Chudley AE (1981) In vivo exposure to diagnostic ultrasound and in vitro assay of sister chromatid exchanges in cultured amniotic fluid cells. ICRS J Med Sci 9:491
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Brulfert, A., Ciaravino, V., Miller, M.W. et al. Diagnostic insonation of extra utero human placentas: No effect of lymphocytic sister chromatid exchange. Hum Genet 66, 289–291 (1984). https://doi.org/10.1007/BF00287629
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DOI: https://doi.org/10.1007/BF00287629