Summary
Genotyping of mitochondrial aldehyde dehydrogenase (ALDH I) was performed in enzymatically amplified DNA of 20 Chinese, Japanese and South Korean families (85 individuals) and in 113 unrelated persons by employing allele-specific oligonucleotide probes and dot blot hybridization. Genotyping individuals with phenotypic deficiency of ALDH I activity always showed the presence of at least one mutant allele. The data are compatible with a model assuming dominant inheritance of the mutant allele, which we have previously suggested on the basis of a population study.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blin N, Stafford DW (1976) A general method for the isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res 3:2303–2308
Braun T, Bober E, Singh S, Agarwal DP, Goedde HW (1987) Evidence for a signal peptide at the amino-terminal end of human mitochondrial aldehyde dehydrogenase. FEBS Lett 215:233–236
Crabb DW, Edenberg HJ, Bosron SF, Li T-K (1989) Genotypes of aldehyde dehydrogenase deficiency and alcohol sensitivity. The inactive ALDH22 allele is dominant. J Clin Invest 83:314–316
Goedde HW, Agarwal DP (1987) Polymorphism of aldehyde dehydrogenase and alcohol sensitivity. Enzyme 37:29–44
Goedde HW, Harada S, Agarwal DP (1979) Racial differences in alcohol sensitivity: a new hypothesis. Hum Genet 51:331–334
Goedde HW, Agarwal DP, Harada S (1980) Genetic studies on alcohol metabolizing enzymes: detection of isozymes in human hair roots. Enzyme 25:281–286
Goedde HW, Agarwal DP, Harada S, Meier-Tackmann D, Ruofu D, Bienzle U, Kroeger A, Hussein L (1983) Population genetic studies on aldehyde dehydrogenase isozyme deficiency and alcohol sensitivity. Am J Hum Genet 35:769–772
Goedde HW, Agarwal DP, Eckey R, Harada S (1985) Population genetic and family studies on aldehyde dehydrogenase deficiency and alcohol sensitivity. Alcohol 2:283–289
Goedde HW, Agarwal DP, Harada S, Whittaker JO, Rothhammer F, Lisker R (1986) Aldehyde dehydrogenase polymorphism in North American, South American and Mexican Indians. Am J Hum Genet 38:395–399
Goedde HW, Singh S, Agarwal DP, Fritze G, Stapel K, Paik YK (1989) Genotyping of mitochondrial aldehyde dehydrogenase in blood samples using allele-specific oligonucleotides: comparison with phenotyping in hair roots. Hum Genet 81:305–307
Harada S, Agarwal DP, Goedde HW (1981) Aldehyde dehydrogenase deficiency as cause of facial flushing reaction to alcohol in Japanese. Lancet II:982
Hempel J, Kaiser R, Jörnvall H (1985) Mitochondrial aldehyde dehydrogenase from human liver: primary structure, differences in relation to the cytosolic enzyme and functional correlations. Eur J Biochem 153:13–28
Hsu LC, Tani K, Fujiyoshi T, Kurachi K, Yoshida A (1985) Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2. Proc Natl Acad Sci USA 82:3771–3775
Hsu LC, Bendel RE, Yoshida A (1987) Direct detection of usual and atypical alleles on the human aldehyde dehydrogenase-2 (ALDH2) locus. Am J Hum Genet 41:996–1001
Hsu LC, Bendel RE, Yoshida A (1988) Genomic structure of the human mitochondrial aldehyde dehydrogenase gene. Genomics 2:57–65
Nagoshi CT, Dixon LK, Johnson RC, Yuen SHL (1988) Familial transmission of alcohol consumption and the flushing response to alcohol in three Oriental groups. J Stud Alcohol 49:261–267
Schwitters SY, Johnson RC, Johnson SB, Ahren FM (1982) Familial resemblences in flushing following alcohol use. Behav Genet 12:349–352
Shibuya A, Yoshida A (1988) Frequency of the atypical aldehyde dehydrogenase-2 gene (ALDH 22 ) in Japanese and Caucasians. Am J Hum Genet 43:741–743
Yoshida A (1983) Differences in the enzymes involved in alcohol metabolism between Caucasians and Orientals. In: Rattazzi MC, Scandalios JG, Whitt GS (eds) Isozymes: current topics in biological and medical research, vol 8. Liss, New York, pp 245–261
Yoshida A, Wang G, Davé V (1983) Determination of genotypes of human liver aldehyde dehydrogenase ALDH2 locus. Am J Hum Genet 35:1107–1116
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Singh, S., Fritze, G., Fang, B. et al. Inheritance of mitochondrial aldehyde dehydrogenase: genotyping in Chinese, Japanese and South Korean families reveals dominance of the mutant allele. Hum Genet 83, 119–121 (1989). https://doi.org/10.1007/BF00286702
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00286702