Conclusions
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1.
Although certain features distinguished ELISA and radiobinding systems, there was considerable inter-laboratory variation in both. As long as such variation persists, assays should be considered individually rather than grouped according to type.
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2.
The assays showed less concordance when measuring insulin autoantibodies than insulin antibodies from insulin treated diabetic patients.
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3.
Non-specific binding was a very important source of variation in some assays (ELISA as well as radiobinding assays).
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4.
Specificity for human insulin was clearly demonstrated in some IAA sera.
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5.
The observed variation in IAA assays could account for the differences in published reports on the frequency and associations of IAA.
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Wilkin, T., Palmer, J., Bonifacio, E. et al. First international workshop on the standardisation of insulin autoantibodies. Diabetologia 30, 676–677 (1987). https://doi.org/10.1007/BF00277328
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DOI: https://doi.org/10.1007/BF00277328