Summary
To investigate the intracellular localization of endothelin in cultured endothelial cells, an immunocytochemical study was carried out by the post-embedding protein A-gold technique with endothelin-specific antiserum. Gold particles were seen on the rough endoplasmic reticulum, the Golgi cisternae, the Golgi vesicles, small vesicles beneath the cell membrane, and the lysosomes. By contrast, no secretory granules were observed. These results suggest that endothelin is secreted by a constitutive pathway and that the lysosome may play an important role in regulating the biological activity of endothelin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ando K, Hirata Y, Shichiri M, Emori T, Marumo F (1989) Presence of immunoreactive endothelin in human plasma. FEBS Lett 245:164–166
Bendayan M, Zollinger M (1983) Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold techniques. J Histochem Cytochem 31:101–109
De Duve C (1969) Combs RRA; Lysosomes in tissue damage mediated by allergic reactions. In: Dingle JT, Fell HB (eds) Lysosomes in biology and pathology, vol 14. Frontiers of biology. North-Holland, Amsterdam, pp 3–40
Hirata Y, Yoshimi H, Takaichi S, Yanagisawa M, Masaki T (1988) Binding and receptor down-regulation of a novel vasoconstrictor endothelin in cultured rat vascular smooth muscle cells. FEBS Lett 239:13–17
Johnson UG, Porter KR (1968) Fine structure of cell division in Chlamydomonas reinhardi. Basal bodies and microtubules. J Cell Biol 38:403–425
Leak LV (1980) Blood Vessels. In: Johannessen JV (ed) Electron microscopy in human medicine, vol 5. McGraw-Hill, New York, pp 87–154
Maldonado CA, Saggau W, Forssmann WG (1986) Cardiodilatin-immunoreactivity in specific atrial granules of human heart revealed by the immunogold stain. Anat Embryol 173:295–298
Naruse M, Naruse K, Kurimoto F, Horiuchi J, Tsuchiya K, Kawana M, Kato Y, Zeng Z-P, Sakurai H, Demura H, Shizume K (1989) Radioimmunoassay for endothelin and immunoreactive endothelin in culture medium of bovine endothelial cells. Biochem Biophys Res Commun 160:662–668
Novikoff AB, Holtzman E (1976) Cells and organelles. Holt, Rinehart, and Winston, New York, pp 143
Reynolds E (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:208–212
Tartakoff A, Vassalli P (1978) Comparative studies of intracellular transport of secretory proteins. J Cell Biol 79:694–707
Uemura H, Naruse M, Hirohama T, Nakamura S, Kasuya Y, Aoto T (1990) Immunoreactive atrial natriuretic peptide in the fish heart and blood plasma examined by electron microscopy, immunohistochemistry and radioimmunoassay. Cell Tissue Res 260:235–247
Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T (1988) A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332:411–415
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nakamura, S., Naruse, M., Naruse, K. et al. Immunocytochemical localization of endothelin in cultured bovine endothelial cells. Histochemistry 94, 475–477 (1990). https://doi.org/10.1007/BF00272609
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00272609