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Inhibition of DNA synthesis and cell division in Salmonella typhimurium by azide

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Summary

Evidence has been obtained that sodium azide is an inhibitor of cell division in wild-type and aziA strains of Salmonella typhimurium. The bacteria grown in media containing sodium azide and glucose formed long filaments. It has been found that sodium azide had a stronger inhibitory effect on DNA synthesis than on cell mass increase. When filaments produced by azide action were transferred to azide-free medium very rapid increase in DNA content was observed during the first 45 min. After this time, when relative DNA content was increased the rate of DNA synthesis was reduced and cell divisions reappeared.

Inhibitory effect of azide on DNA biosynthesis in vitro was observed with toluenized cells of S. typhimurium. Only ATP-dependent radioactive dTMP incorporation into DNA was affected by sodium azide. It had no effect on the incorporation in the absence of ATP.

Mutant aziC was isolated in S. typhimurium by scoring for clones with normal cell division in the presence of sodium azide. Azide had much less effect on DNA biosynthesis in vivo and in vitro in aziC cells as compared with isogenic controls.

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References

  • Bogucka, K., Wojtczak, L.: Effect of sodium azide on oxidation and phosphorylation processes in rat-liver mitochondria. Biochim. biophys. Acta (Amst.) 122, 381–392 (1966)

    Google Scholar 

  • Burton, K.: A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62, 315–323 (1956)

    Google Scholar 

  • Cairns, J., Denhardt, D. T.: Effect of cyanide and carbon monoxide on the replication of bacterial DNA in vivo. J. molec. Biol. 36, 335–324 (1968)

    Google Scholar 

  • Cieśla, Z., Bagdasarian, M., Szczurkiewicz, W., Pryzgońska, M., Klopotowski, T.: Defective cell division in thermosensitive mutants of Salmonella typhimurium. Molec. gen. Genet. 116, 107–125 (1972)

    Google Scholar 

  • Clark, D. J.: The regulation of DNA replication and cell division in E. coli B/r. Cold Spr. Harb. Symp. quant. Biol. 33, 823–838 (1968)

    Google Scholar 

  • Goss, W. A., Deitz, W. H., Cook, T. M.: Mechanism of action of nalidixic acid on Escherichia coli. II. Inhibition of deoxyribonucleic acid synthesis. J. Bact. 89, 1068–1074 (1965)

    Google Scholar 

  • Kornberg, T., Gefter, M. L.: Purification and DNA synthesis in cell-free extracts: Properties of DNA polymerase II. Proc. nat. Acad. Sci. (Wash.) 68, 768–764 (1971)

    Google Scholar 

  • Mordoh, J., Hirota, Y., Jacob, F.: On the process of cellular division in Escherichia coli, V. Incorporation of deoxynucleoside triphosphates by DNA thermosensitive mutants of Escherichia coli also lacking polymerase activity. Proc. nat. Acad. Sci. (Wash.) 67, 773–778 (1970)

    Google Scholar 

  • Moses, R. E., Richardson, Ch. C.: Replication and repair of DNA in cells of Escherichia coli treated with toluene. Proc. nat. Acad. Sci. (Wash.) 67, 674–681 (1970)

    Google Scholar 

  • Nilan, R. A., Sideris, E. G., Kleinhofs, A., Sander, C., Konzak, C. F.: Azide—a potent mutagen. Mutation Res. 17, 142–144 (1973)

    Google Scholar 

  • Pisetsky, D., Berkower, I., Wickner, R., Hurwitz, J.: Role of ATP in DNA synthesis in Escherichia coli. J. molec. Biol. 71, 557–571 (1972)

    Google Scholar 

  • Schaller, H., Otto, B., Nüsslein, V., Hut, J., Herrmann, R., Bonhoeffer, F.: Deoxyribonucleic acid replication in vitro. J. molec. Biol. 63, 183–200 (1972)

    Google Scholar 

  • Smith, L.: Cytochrome systems in aerobic electron transport in the bacteria. Gunsalus, T. C. and Stanier, R. Y. (eds.), vol. 2, p. 365–396. New York and London: Academic Press (1961)

    Google Scholar 

  • Virasoro, S., Mordoh, J., Hirota, Y.: Further studies on DNA thermosensitive mutants of Escherichia coli using the toluenized cell system. Biochimie 56, 363–371 (1974)

    Google Scholar 

  • Whitfield, H. J., Levine, G.: Isolation and characterization of a mutant of Salmonella typhimurium deficient in a major deoxyribonucleic acid polymerase activity. J. Bact. 116, 54–58 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

    Z. Cieśla, Krystyna Mardarowicz & T. Klopotowski

Authors
  1. Z. Cieśla
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  2. Krystyna Mardarowicz
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  3. T. Klopotowski
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Additional information

Communicated by F. Kaudewitz

This work was supported by the Polish Academy of Sciences within the project 09.3.1., and by the U.S. Public Health Service, grant No. 05-032-1.

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Cieśla, Z., Mardarowicz, K. & Klopotowski, T. Inhibition of DNA synthesis and cell division in Salmonella typhimurium by azide. Molec. Gen. Genet. 135, 339–348 (1974). https://doi.org/10.1007/BF00271148

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  • DOI: https://doi.org/10.1007/BF00271148

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