Abstract
A two-layer culture method was established that uses an organic solvent to remove shikonin derivatives produced on cell surfaces during the culture of suspension cells of Lithospermum erythrorhizon. Some paraffins and a fatty acid ester made suitable solvents, whereas olefins and aromatic solvents extensively inhibited the production of shikonin derivatives. The yield of derivatives increased with an increase in the carbon chain length of the n-paraffin used as the solvent and when the oxygen supply was sufficient it reached the value found for the ordinary culture method.
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Communicated by M. Tabata
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Deno, H., Suga, C., Morimoto, T. et al. Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon . Plant Cell Reports 6, 197–199 (1987). https://doi.org/10.1007/BF00268478
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DOI: https://doi.org/10.1007/BF00268478