Summary
The relationship between the structures of diverse heterocyclic nitrogen (N) compounds and the effectiveness of these compounds for the inhibition of nitrification in soil was studied by determining the effects of different amounts of 12 unsubstituted and 33 substituted heterocyclic N compounds on the production of (NO −2 +NO −3 )-N in soils incubated at 25 °C for 21 days after treatment with ammonium sulfate. The results showed that unsubstituted heterocyclic N compounds containing two adjacent ring N atoms inhibit nitrification in soil and that two of these compounds, pyrazole and 1,2,4-triazole, are potent inhibitors. They also showed that several substituted pyrazoles and thiadiazoles are good inhibitors of nitrification in soil (e.g., 3-methylpyrazole and 3,4-dichloro-1,2,5-thiadiazole).
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References
Bédard C, Knowles R (1989) Physiology, biochemistry, and specific inhibitors of CH4, NH +4 , and CO oxidation by methanotrophs and nitrifiers. Microbiol Rev 53:68–84
Blomstrand R, Östling-Wintzell H, Löf A, McMartin K, Tolf BR, Hedström KG (1979) Pyrazoles as inhibitors of alcohol oxidation and as important tools in alcohol research: An approach to therapy against methanol poisoning. Proc Natl Acad Sci USA 76:3499–3503
Bremner JM (1965) Total nitrogen. In: Black CA et al. (eds) Methods of soil analysis: Part 2. Am Soc Agron, Madison, Wise, Agronomy 9:1149–1178
Bremner JM, Keeney DR (1966) Determination and isotope-ratio analysis of different forms of nitrogen in soils: 3. Exchangeable ammonium, nitrate, and nitrite by extraction-distallation methods. Soil Sci Soc Am Proc 30:577–582
Bremner JM, Blackmer AM, Bundy LG (1978) Problems in use of nitrapyrin (N-Serve) to inhibit nitrification in soils. Soil Biol Biochem 10:441–442
Bundy LG, Bremner JM (1972) A simple titrimetric method for determination of inorganic carbon in soils. Soil Sci Soc Am Proc 36:273–275
Bundy LG, Bremner JM (1973) Inhibition of nitrification in soils. Soil Sci Soc Am Proc 37:396–398
Colombo G, Villafranca JJ (1984) An acetylenic mechanism-based inhibitor of dopamine β-hydroxylase. J Biol Chem 259: 15017–15020
Genrich DA, Bremner JM (1972) A reevaluation of the ultrasonicvibration method of dispersing soils. Soil Sci Soc Am Proc 36:944–947
Goring CAI, Laskowski DA (1982) The effects of pesticides on nitrogen transformations in soils. In: Stevenson FJ et al. (eds) Nitrogen in agricultural soils. Am Soc Agron, Madison, Wise, Agronomy 22:689–714
Gilchrist TL (1985) Heterocyclic chemistry. Pitman, London
Hauck RD (1980) Mode of action of nitrification inhibitors. In: Stelly M et al. (eds) Nitrification inhibitors — potentials and limitations. ASA Spec Publ 38, Am Soc Agron, Madison, Wise, pp 19–32
Hauck RD (1984) Technological approaches to improving the efficiency of nitrogen fertilizer use by crop plants. In: Hauck RD et al. (eds) Nitrogen in crop production. Am Soc Agron, Madison, Wisc, pp 551–560
Hyman MR, Wood PM (1985) Suicidal labelling and inactivation of ammonia mono-oxygenase by acetylene. Biochem J 227:719–725
Hyman MR, Sansome-Smith AW, Shears JH, Wood PM (1985) A kinetic study of benzene oxidation to phenol by whole cells of Nitrosomonas europaea and evidence for the further oxidation of phenol to hydroquinone. Arch Microbiol 143:302–306
McCarty GW, Bremner JM (1986) Inhibition of nitrification in soil by acetylenic compounds. Soil Soc Soc Am J 50:1198–1201
Mebius LJ (1960) A rapid method for determination of organic carbon in soil. Anal Chim Acta 22:120–124
Oremland RS, Capone DG (1988) Use of “specific” inhibitors in biogeochemistry and microbial ecology. In: Marshall KC (ed) Advances in microbial ecology, vol. 10. Plenum Press, New York, pp 285–383
Ortiz de Montellano PR, Kunze KL (1980) Self-catalyzed inactivation of hepatic cytochrome P-450 by ethynyl substrates. J Biol Chem 255:5578–5585
Powell SJ, Prosser JI (1985) The effect of nitrapyrin and chloropicolinic acid on ammonium oxidation by Nitrosomonas europaea. FEMS Microbiol Lett 28:51–54
Salvas PL, Taylor BF (1984) Effect of pyridine compounds on ammonia oxidation by autotrophic nitrifying bacteria and Methylosinus trichosporium OB3b. Curr Microbiol 10:53–56
Slangen JHG, Kerkhoff P (1984) Nitrification inhibitors in agriculture and horticulture: A literature review. Fert Res 5:1–76
Suzuki I, Dular U, Kwok SC (1974) Ammonia or ammonium ion as substrate for oxidation by Nitrosomonas europaea cells and extracts. J Bacteriol 120:556–558
Theorell H, Yonetani T, Sjoberg B (1969) On the effects of some heterocyclic compounds on the enzymic activity of liver alcohol dehydrogenase. Acta Chem Scand 23:255–260
Topp E, Knowles R (1984) Effects of nitrapyrin [2-chloro-6-(trichloromethyl)pyridinej on the obligate methanotroph Methylosinus trichosporium OB3b. Appl Environ Microbiol 47:258–262
Walter R, Hartbrich HJ, Lang S, Jasche K, Jumar A, Klepel K, Thieme MK, Günther K, Moritz C (1985) Erste Ergebnisse zur nitrifiziden Wirksamkeit von 1-carbamoyl-3(5)-methypyrazol (CMP). Speziell Agrochemie, Forsch Praxis 14:5–14
White INH (1978) Metabolic activation of acetylene substituents to derivatives in the rat causing the loss of hepatic cytochrome P-450 and haem. Biochem J 174:853–861
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McCarty, G.W., Bremner, J.M. Inhibition of nitrification in soil by heterocyclic nitrogen compounds. Biol Fert Soils 8, 204–211 (1989). https://doi.org/10.1007/BF00266480
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DOI: https://doi.org/10.1007/BF00266480