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Seasonal changes in phagocytic capacity and superoxide anion production of blood phagocytes from tench (Tinca tinca, L.)

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Abstract

Seasonal variations in the ex vivo phagocytic function of blood cells from tench, including ingestion capacity of inert particles and its destruction (microbicide capacity) assessed by measurement of superoxide anion production, were studied. Tench were maintained under natural conditions throughout the year, and the different assays of samples taken during each season were initially performed in vitro at 22°C and the results compared. Subsequently, assays were performed at the same temperature as that of the water ponds in which the fish were kept (“seasonal temperature”: 12°C in winter, 22°C in spring and autumn and 30°C in summer) and the results compared seasonally. The results at 22°C showed that phagocytic capacity was greatest in spring and summer and lowest in winter. However, when phagocytic capacity was measured at seasonal temperature, highest values appeared in winter and lowest in summer and autumn. Nitroblue tetrazolium reduction by tench phagocytes after phagocytosing latex beads demonstrated a similar seasonal behaviour at both 22°C in each season and at seasonal temperature. The highest values appeared in summer, which suggests a better microbicide capacity in this season. The results obtained in this study suggest that for a correct interpretation of ex vivo phagocytic capacity of fish through the year it is necessary to use the same assay temperature as that of the water in which the fish is kept.

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Abbreviations

MS222:

tricaine methanesulphonate

PBS:

phosphate-buffered saline

NBT:

nitroblue tetrazolium

PEI:

phagocytic efficiency index

PP:

percent phagocytosis

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Authors and Affiliations

  1. Departamento de Fisiologia (Animal), Facultad de Ciencias, Universidad de Extremadura, Avda, Elvas, s/n, E-06071, Badajoz, Spain

    M. E. Collazos, C. Barriga & E. Ortega

Authors
  1. M. E. Collazos
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  2. C. Barriga
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  3. E. Ortega
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Communicated by H. Langer

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Collazos, M.E., Barriga, C. & Ortega, E. Seasonal changes in phagocytic capacity and superoxide anion production of blood phagocytes from tench (Tinca tinca, L.). J Comp Physiol B 165, 71–76 (1995). https://doi.org/10.1007/BF00264688

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