Abstract
When pharate adults of the flesh fly Sarcophaga crassipalpis are exposed to 40°C for 4 h they become more tolerant of high temperatures that are normally lethal (thermotolerance). In contrast, a 1-h exposure to 45°C decreases tolerance to a subsequent high temperature challenge (thermosensitivity). While control flies experience little mortality when held at 35°C for 24–48 h the thermosensitized flies die when exposed to 35°C. Sensitivity to a second thermal challenge slowly decays over a 72-h period. The acquisition of thermotolerance prevents the development of thermosensitivity. Brains from thermosensitized flies cultured at 43°C express the 72-kDa heat-shock protein and normal protein synthesis is inhibited. This implies that development of thermosensitivity is not associated with a loss in the capacity to express the 72-kDa heat-shock protein.
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Abbreviations
- ICN:
-
ICN Biomedicals, Inc. PO Box 19536, Irvine, CA 92713-9921
- LD light:
-
dark cycle
- LT50 :
-
time required to kill 50% of the test animals
- SDS:
-
sodium dodecyl sulfate
- TRIS:
-
Tris(hydroxymethyl)aminomethane
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Yocum, G.D., Denlinger, D.L. Induction and decay of thermosensitivity in the flesh fly, Sarcophaga crassipalpis . J Comp Physiol B 163, 113–117 (1993). https://doi.org/10.1007/BF00263595
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DOI: https://doi.org/10.1007/BF00263595