Summary
A quantitative electron microscopic investigation of the nucleus interpositus in cat cerebellum reveals that about 1.5% of all observed synapses are established between synaptic vesicle-bearing profiles. It is shown by serial sections that 70% of these synaptic complexes are triadic arrangements and 30% are serial synapses. Further analysis discloses that the first presynaptic element in the triadic and serial synapses may be one of four different axonal types: (A) Purkinje-cell axons; (B) and (C) afferent fibers containing large round vesicles and originating from the brain stem (probably mossy and climbing fiber collaterals); and (D) axon terminals containing small round vesicles. Indirect evidence suggests that type D profiles are the recurrent axon collaterals of the projective neurons. The second, postsynaptic and presynaptic, vesicle-bearing process in these complexes is either a class D terminal, or a somewhat more “dendrite-like” profile (Class E) containing flattened vesicles, and identified as belonging to processes of local Golgi type II interneurons.
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Andres, K.H.: Der Feinbau des Bulbus olfactorius der Ratte unter besonderer Berücksichtigung der synaptischen Verbindungen. Z. Zellforsch. 65, 530–561 (1965)
Angaut, P., Repérant, J.: Fine structure of the optic fibre termination layers in the pegeon optic tectum: a Golgi and electron microscope study. Neuroscience 1, 93–105 (1976)
Angaut, P., Sotelo, C.: The fine structure of the cerebellar central nuclei in the cat. II. Synaptic organization. Exp. Brain Res. 16, 431–454 (1973)
Chan-Palay, V.: On the identification of the afferent axon terminals in the nucleus lateralis of the cerebellum. An electron microscope study. Z. Anat. Entwickl.-Gesch. 142, 149–186 (1973a)
Chan-Palay, V.: Axon terminals of the intrinsic neurons in the nucleus lateralis of the cerebellum. An electron microscope study. Z. Anat. Entwickl.-Gesch. 142, 187–206 (1973b)
Chan-Palay, V.: Neuronal circuitry in the nucleus lateralis of the cerebellum. Z. Anat. Entwickl.-Gesch. 142, 259–265 (1973c)
Colonnier, M., Guillery, R.W.: Synaptic organization in the lateral geniculate nucleus of the monkey. Z. Zellforsch. 62, 333–355 (1964)
Dowling, J.E., Boycott, B.B.: Organization of the primate retina: electron microscopy. Proc. Roy. Soc. B. 166, 80–111 (1966)
Eccles, J.C., Ito, M., Szentágothai, J.: The cerebellum as a neuronal machine. Berlin-Heidelberg-New York: Springer 1967
Famiglietti, E.V., Jr.: Dendro-dendritic synapses in the lateral geniculate nucleus of the cat. Brain Res. 20, 181–191 (1970)
Famiglietti, E.V., Jr., Peters, A.: The synaptic glomerulus and the intrinsic neuron in the dorsal lateral geniculate nucleus of the cat. J. comp. Neurol. 144, 285–334 (1972)
Gray, E.G.: A morphological basis for pre-synaptic inhibition. Nature (Lond.) 193, 82–83 (1962)
Gray, E.G.: Electron microscopy of presynaptic organelles of the spinal cord. J. Anat. (Lond.) 97, 101–106 (1963)
Hajdu, F., Somogyi, Gy., Tömböl, T.: Neuronal and synaptic arrangement in the lateralis posterior-pulvinar complex of the thalamus. Brain Res. 73, 89–104 (1974)
Halász, B., Pupp, L.: Hormone secretion of the anterior pituitary gland after physical interruption of all nervous pathways to the hypophysiotrophic area. Endocrinology 77, 553–562 (1965)
Hámori, J.: Presynaptic-to-presynaptic axon contacts under experimental conditions giving rise to rearrangement of synaptic structures. In: Structure and Function of Inhibitory Neuronal Mechanisms (ed. C. von Euler), pp. 71–80. Oxford-New York: Pergamon Press 1968
Hámori, J., Pasik, T., Pasik, P., Szentágothai, J.: Triadic synaptic arrangements and their possible significance in the lateral geniculate nucleus of the monkey. Brain Res. 80, 379–393 (1974)
Harding, B.N.: Dendro-dendritic synapses, including reciprocal synapses, in the ventro-lateral nucleus of the monkey thalamus. Brain Res. 34, 181–185 (1971)
Hopsu, V.K., Arstila, A.U.: An apparent somato-somatic synaptic structure in the pineal gland of the rat. Exp. Cell Res. 37, 484–487 (1965)
Lábos, E.: On the role of synaptic arrangements with presynaptic dendrites and of Golgi neuronal interconnections in sensory relay nuclei. In: Neuron Concept Today (eds. J. Szentágothai, J. Hámori and S. Vizy), pp. 299–300. Budapest: Akadémiai Kiadó 1977
Lieberman, A.R.: Neurons with presynaptic perikarya and presynaptic dendrites in the rat lateral geniculate nucleus. Brain Res. 59, 35–39 (1973)
Lieberman, A.R., Webster, K.E.: Presynaptic dendrites and a distinctive class of synaptic vesicle in the rat dorsal lateral geniculate nucleus. Brain Res. 42, 196–200 (1972)
Lieberman, A.R., Webster, K.E.: Aspects of the synaptic organization of intrinsic neurons in the dorsal lateral geniculate nucleus. An ultrastructural study of the normal and of the experimentally deafferented nucleus in the rat. J. Neurocytol. 3, 677–710 (1974)
Lund, R.D.: Synaptic patterns of the superficial layers of the superior colliculus of the rat. J. comp. Neurol. 135, 179–208 (1969)
Majorossy, K., Kiss, A.: Specific patterns of neuron arrangement and of synaptic articulation in the medial geniculate body. Exp. Brain Res. 26, 1–17 (1976)
Matsushita, M., Iwahori, N.: Structural organization of the interpositus and dentate nuclei. Brain Res. 35, 17–36 (1971)
Morest, D.K.: Dendro-dendritic synapses of cells that have axons: The fine structure of the Golgi type II cell in the medial geniculate body of the cat. Z. Anat. Entwickl.-Gesch. 133, 216–246 (1971)
Palay, S.L., Chan-Palay, V.: Cerebellar cortex. Cytology and organization. Berlin-Heidelberg-New York: Springer 1974
Palkovits, M., Mezey, E., Hámori, J., Szentágothai, J.: Quantitative histological analysis of the cerebellar nuclei in the cat. I. Numerical data on cells and on synapses. Exp. Brain Res. 28, 189–209 (1977)
Pappas, G.D., Cohen, E.B., Purpura, D.P.: Fine structure of synaptic and non-synaptic neuronal relations in the thalamus of the cat. In: The Thalamus (eds. D.P. Purpura and M.D. Yahr), pp. 47–75. New York: Columbia Univ. Press 1964
Pasik, P., Pasik, T., Hámori, J.: Synapses between interneurons in the lateral geniculate nucleus of monkeys. Exp. Brain Res. 25, 1–13 (1976)
Pasik, P., Pasik, T., Hámori, J., Szentágothai, J.: Golgi II type interneurons in the neuronal circuit of the monkey lateral geniculate nucleus. Exp. Brain Res. 17, 18–34 (1973)
Price, J.L.: The termination of centrifugal fibres in the olfactory bulb. Brain Res. 7, 483–486 (1968)
Rall, W., Shepherd, G.M., Reese, T.S., Brightman, M.W.: Dendro-dendritic synaptic pathway for inhibition in the olfactory bulb. Exp. Neurol. 14, 44–46 (1966)
Ralston, H.J. III: The organization of the substantia gelatinosa Rolandi in the cat lumbosacral spinal cord. Z. Zellforsch. 67, 1–23 (1965)
Ralston, H.J. III.: Evidence for presynaptic dendrites and a proposal for their mechanism of action. Nature (Lond.) 230, 585–587 (1971)
Sétaló, G., Székely, G.: The presence of membrane specializations indicative of somato-dendritic synaptic junctions in the optic tectum of the frog. Exp. Brain Res. 4, 237–242 (1967)
Sloper, J.J.: Dendro-dendritic synapses in the primate motor cortex. Brain Res. 34, 186–192 (1971)
Szentágothai, J., Hámori, J., Tömböl, T.: Degeneration and electron microscope analysis of the synaptic glomeruli in the lateral geniculate body. Exp. Brain Res. 2, 283–301 (1966)
Valverde, F.: The pyramidal tract in rodents. A study of its relations with the posterior column nuclei, dorsolateral reticular formation of the medulla oblongata, and cervical spinal cord. Z. Zellforsch. 71, 297–363 (1966)
Walberg, F.: Axoaxonic contacts in the cuneate nucleus, probable basis for presynaptic depolarization. Exp. Neurol. 13, 218–231 (1963)
Wong, M.T.T.: Somato-dendritic and dendro-dendritic synapses in the squirrel monkey lateral geniculate nucleus. Brain Res. 20, 135–139 (1970)
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Hámori, J., Mezey, É. Serial and triadic synapses in the cerebellar nuclei of the cat. Exp Brain Res 30, 259–273 (1977). https://doi.org/10.1007/BF00237255
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DOI: https://doi.org/10.1007/BF00237255