Abstract
Plant regeneration of Actinidia deliciosa var. deliciosa cv. Hayword was obtained from protoplasts isolated from petiole derived long-term callus cultures. Protoplasts were cultured in liquid medium over agarose gelled medium. Regenerated green callus, plated on solid medium, could develop shoots that rooted spontaneously in hormone-less medium. The plants obtained are growing fast in soil and present a normal phenotype.
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Abbreviations
- BAP:
-
benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- DTT:
-
dithiotreitol
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- Kin:
-
kinetin
- MES:
-
2-(N-morpholino) ethanesulphonic acid
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
naphthalene-1-acetic acid
- SH:
-
Schenk and Hildebrandt (1972) medium
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Communicated by I. Potrykus
This Research was supported by JNICT and INIC
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Oliveira, M.M., Pais, M.S.S. Plant regeneration from protoplasts of long-term callus cultures of Actinidia deliciosa var. deliciosa cv. Hayword (Kiwifruit). Plant Cell Reports 9, 643–646 (1991). https://doi.org/10.1007/BF00231807
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DOI: https://doi.org/10.1007/BF00231807