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Molecular markers (RFLPs and HSPs) for the genetic dissection of thermotolerance in maize

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Summary

Cellular membrane stability (CMS) is a physiological index widely used to evaluate thermostability in plants. The genetic basis of the character has been studied following two different approaches: restriction fragment length polymorphism (RFLP) analysis, and the effects of segregating heat shock protein (HSP) loci. RFLP analysis was based on a set of recombinant inbreds derived from the T32 × CM37 F1 hybrid and characterized for about 200 RFLP loci. Heritability of CMS estimated by standard quantitative analysis was 0.73. Regression analysis of CMS on RFLPs detected a minimum number of six quantitative trait loci (QTL) accounting for 53% of the genetic variability. The analysis of the matrices of correlation between RFLP loci, either within or between chromosomes, indicates that no false assignment was produced by this analysis. The effect of HSPs on the variability of the CMS was tested for a low-molecular-weight peptide (HSP-17) showing presence-absence of segregation in the B73 × Pa33 F2 population. Although the genetic variability of the character was very high (h 2=0.58) the effect of HSP-17 was not significant, indicating either that the polypeptide is not involved in the determination of the character or that its effect is not statistically detectable.

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References

  • Anderson RL, Bancroft TA (1952) Statistical theory in research. McGraw-Hill, New York

    Google Scholar 

  • Bewley JD (1979) Physiological aspects of desiccation tolerance. Annu Rev Plant Physiol 30:195–238

    Article  MATH  Google Scholar 

  • Blum A (1985) Breeding crop varieties for stress environments. CRC Crit Rev Plant Sci 3:199–238

    Google Scholar 

  • Blum A (1986) Methods of plant breeding for drought resistance. In: Monti L, Porceddu E (eds) Drought resistance in plants. EEC, Amalfi, Italy, pp 235–254

    Google Scholar 

  • Blum A, Ebercon A (1981) Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci 21:43–47

    Google Scholar 

  • Burr B, Burr F (1988) I polimorfismi dei frammenti generati da enzimi di restrizione e il loro impiego nel miglioramento genetico. Agric Ric 93:61–68

    Google Scholar 

  • Burr B, Burr FA, Thompson KH, Albertson CM, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526

    CAS  PubMed  Google Scholar 

  • Edwards MD, Stuber CW, Wendel JF (1987) Molecular marker-facilitated investigations of quantitative trait loci in maize. I. Numbers, genomic distribution, and types of gene action. Genetics 116:113–125

    CAS  PubMed  Google Scholar 

  • Frova C, Taramino G, Binelli G, Ottaviano E (1988) Heat-shock protein variability in maize. Maydica 33:65–76

    Google Scholar 

  • Frova C, Taramino G, Binelli G (1989) Heat shock proteins during pollen development in maize. Dev Genet 10:324–332

    Google Scholar 

  • Ganal MW, Young ND, Tanksley SD (1989) Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400

    Google Scholar 

  • Helentjaris T, Burr B (1989) Development and application of molecular markers to problems in plant genetics. Current communications in molecular biology. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY, pp 160

    Google Scholar 

  • Jones AR, Qualset CO (1984) Breeding crops for environmental stress tolerance. In: Collins GB, Petolino JG (eds) Applications of genetic engineering to crop improvement. Nijhoff M, Junk W Publ, Dordrecht, pp 305–340

    Google Scholar 

  • Kahler AL, Wehrhahn CF (1986) Association between quantitative traits and enzyme loci in the F2 population of a maize hybrid. Theor Appl Genet 72:15–26

    Google Scholar 

  • Krishnan M, Nguyen HT, Burke JJ (1989) Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiol 90:140–145

    Google Scholar 

  • Levitt J (1980) Responses of plants to environmental stresses. Chilling, freezing, and high temperature stresses. Academic Press, New York

    Google Scholar 

  • Lin CY, Roberts JK, Key JL (1984) Acquisition of thermotolerance in soybean seedlings. Synthesis and accumulation of heat shock proteins and their cellular localization. Plant Physiol 74:152–160

    Google Scholar 

  • Lindquist S (1986) The heat shock response. Annu Rev Biochem 55:1151–1191

    Article  CAS  PubMed  Google Scholar 

  • Mansfield MA, Key JL (1987) Synthesis of the low-molecular-weight heat shock proteins in plants. Plant Physiol 84:1007–1017

    Google Scholar 

  • Martin B, Nienhuis J, King G, Schaefer A (1989) Restriction fragment length polymorphisms associated with water use efficiency in tomato. Science 243:1725–1728

    Google Scholar 

  • Martineau JR, Specht JE, Williams JH, Sullivan CY (1979a) Temperature tolerance in soybeans. I. Evaluation of a technique for assessing cellular membrane thermostability. Crop Sci 19:75–78

    Google Scholar 

  • Martineau JR, Williams JH, Specht JE (1979b) Temperature tolerance in soybeans. II. Evaluation of segregating population for membrane thermostability. Crop Sci 19:79–82

    Google Scholar 

  • Mascarenhas JP (1984) Molecular mechanisms of heat stress tolerance. In: Collins GB, Petolino JG (eds) Applications of genetic engineering to crop improvement. Nijhoff M, Junk W Publ, Dordrecht, pp 391–425

    Google Scholar 

  • McDaniel RG (1982) The physiology of temperature effects on plants. In: Christiansen MN, Lewis CF (eds) Breeding plants for less favorable environments. Wiley and Sons, New York, pp 13–45

    Google Scholar 

  • McWilliams JR (1980) Adaptation to water and high temperature stress: summary and synthesis — adaptation to high temperature stress. In: Turner NC, Kramer PJ (eds) Adaptation of plants to water and high temperature stress. Wiley and Sons, New York, pp 444–447

    Google Scholar 

  • Nienhuis J, Helentjaris T, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphisms analysis of loci associated with insect resistance in tomato (1987) Crop Sci 27:797–803

    Google Scholar 

  • Osborn TC, Alexander DC, Fobes JF (1987) Identification of restriction fragment length polymorphisms linked to genes controlling soluble solids content in tomato fruit. Theor Appl Genet 73:350–356

    Google Scholar 

  • Ottaviano E, Mulcahy DL (1989) Genetics of angiosperm pollen. Adv Genet 26:1–64

    Google Scholar 

  • Ottaviano E, Sari Gorla M (1988) Selecting cultivars for resistance to high and low temperature. In: Biotechnology in tropical crops improvement. ICRISAT, Hyderabad India, pp 97–105

    Google Scholar 

  • Ottaviano E, Sari Gorla M, Mulcahy DL (1990) Pollen selection: efficiency and monitoring. In: Markert C, Oghita Z (eds) Isozymes: structure, function, and use in biology and medicine. Wiley-Liss, New York, pp 575–588

    Google Scholar 

  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726

    Article  CAS  PubMed  Google Scholar 

  • Sachs MM, Ho TD (1986) Alteration of gene expression during environmental stress in plants. Annu Rev Plant Physiol 37:363–376

    Google Scholar 

  • Sullivan CY (1972) Mechanism of heat and drought resistance in grain sorghum and methods of measurement. In: Rao NGP, House LR (eds) Sorghum in the seventies. Oxford and India Book House, New Delhi, pp 247–264

    Google Scholar 

  • Tanksley SD, Hewitt J (1988) Use of molecular markers in breeding for soluble solids content in tomato — a reexamination. Theor Appl Genet 75:811–823

    Google Scholar 

  • Tanksley SD, Medina-Filho H, Rick CM (1982) Use of naturally occurring enzyme variation to detect and map genes controlling quantitative traits in an interspecific backcross of tomato. Heredity 49:11–25

    Google Scholar 

  • Taylor BA (1976) Genetic analysis of susceptibility to isoniazid-induced seizures in mice. Genetics 83:373–377

    Google Scholar 

  • Weller JI, Soller M, Brody T (1988) Linkage analysis of quantitative traits in an interspecific cross of tomato (Lycopersicon esculentum × Lycopersicon pimpinellifolium) by means of genetic markers. Genetics 118:329–339

    Google Scholar 

  • Zamir D, Tanksley SD, Jones AJ (1981) Haploid selection for low temperature tolerance of tomato pollen. Genetics 101:129–137

    Google Scholar 

  • Zivy M (1987) Genetic variability for heat shock proteins in common wheat. Theor Appl Genet 74:209–213

    Google Scholar 

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Communicated by P. L. Pfahler

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Ottaviano, E., Sari Gorla, M., Pè, E. et al. Molecular markers (RFLPs and HSPs) for the genetic dissection of thermotolerance in maize. Theoret. Appl. Genetics 81, 713–719 (1991). https://doi.org/10.1007/BF00224979

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