Summary
Frog pancreatic tissue was pulse-labelled in vitro with 3H-leucine and protein transport was studied in exocrine cells by electron microscope autoradiography. The proteins appeared to be synthesized in the RER and transported to the secretory granules along a similar route and with the same velocity as previously described under in vitro conditions.
Evidence was obtained for the involvement of the vesicular and tubular elements at the periphery of the Golgi system in transferring protein from the RER to the Golgi cisternae.
Kinetics of the release of newly synthesized proteins from the RER and their appearance in the condensing vacuoles are discussed and related to results reported from other tissues.
The transport velocity in this poikilothermic system was studied in relation to the incubation temperature and compared with results reported from its mammalian counterpart. At temperatures between 20 and 30° C intracellular protein transport occurs faster in the frog than in the Guinea pig pancreas. At higher temperature the transport process was severely disturbed in the frog.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amsterdam, A., Jamieson, J.D.: Studies on dispersed pancreatic exocrine cells. II. Functional characsteristics of separated cells. J. Cell Biol. 63, 1057–1073 (1974)
Caro, L.G., Palade, G.E.: Protein synthesis, storage and discharge in the pancreatic exocrine cell. J. Cell Biol. 20, 473–495 (1964)
Castle, J.D., Jamieson, J.D., Palade, G.E.: Radioautographic analysis of the secretory process in the parotid acinar cell of the rabbit. J. Cell Biol. 53, 290–311 (1972)
Flickinger, C.J.: Synthesis, intracellular transport, and release of secretory protein in the seminal vesicle of the rat, as studied by electron microscope radioautography. Anat. Rec. 180, 407–426 (1974a)
Flickinger, C.J.: Protein secretion in the rat ventral prostate and the relation of Golgi vesicles, cisternae and vacuoles, as studied by electron microscope radioautography. Anat. Rec. 180, 427–448 (1974b)
Fujita, H.: Fine structure of the thyroid gland. Int. Rev. Cytol. 40, 197–280 (1975)
Geuze, J.J., Kramer, M.F.: Function of coated membraness and multivesicular bodies during membrane regulation in stimulated exocrine pancreas. Cell Tiss. Res. 156, 1–20 (1974)
Hopkins, C.R.: The biosynthesis, intracellular transport, and packaging of melanocyte-stimulating peptides in the amphibian pars intermedia. J. Cell Biol. 53, 642–653 (1972)
Jamieson, J.D., Palade, G.E.: Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules. J. Cell Biol. 34, 597–615 (1967)
Jamieson, J.D., Palade, G.E.: item IV. Metabolic requirements. J. Cell Biol. 39, 589–603 (1968)
Jamieson, J.D., Palade, G.E.: Condensing vacuole conversion and zymogen granule discharge in pancreatic exocrine cells: metabolic studies. J. Cell Biol. 48, 503–522 (1971)
Kafatos, F.C., Kiortsis, V.: The packaging of a secretory protein. Kinetics of cocoonase zymogen transport into a storage vacuole. J. Cell Biol. 48, 426–431 (1971)
Slot, J.W., Geuze, J.J.: Protein transport through the Golgi system in the frog exocrine pancreas cell. In: Electron microscopy and cytochemistry. (E. Wisse, W.Th. Daems, I. Molenaar and P. van Duyn, eds.). Amsterdam: North-Holland Publishing Company 1973
Slot, J.W., Geuze, J.J., Poort, C.: Synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (Rana esculenta) I. An ultrastructural and autoradiographic study. Cell Tiss. Res. 155, 135–154 (1974)
Venrooij, W.J. van, Poort, C., Kramer, M.F., Jansen, M.T.: Relationship between extracellular amino acids and protein synthesis in vitro in the rat pancreas. Europ. J. Biochem. 30, 427–433 (1972)
Weinstock, A., Leblond, C.P.: Elaboration of the matrix-glycoprotein of enamel by the secretory ameloblasts of the rat incisor as revealed by radioautography after galactose-3H injection. J. Cell Biol. 51, 26–51 (1971)
Weinstock, M., Leblond, C.P.: Synthesis, migration and release of precursor collagen by odontoblasts, as visualized by radioautography after 3H-proline administration. J. Cell Biol. 60, 92–127 (1974)
Zeigel, R.F., Dalton, A.J.: Speculations based on the morphology of the Golgi systems in several types of protein secreting cells. J. Cell Biol. 15, 45–54 (1962)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Slot, J.W., Geuze, J.J. & Poort, C. Synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (Rana esculenta). Cell Tissue Res. 167, 147–165 (1976). https://doi.org/10.1007/BF00224324
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00224324