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Ultrastructure of synaptosomes from one-day old and adult rat brain stem

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Summary

The ultrastructure and protein content of the five subfractions of the crude mitochondrial fraction from the brain stem of the 1-day old and adult rat was examined. The morphological composition of the subfractions after fixation in glutaraldehyde and osmiumtetroxide in the adult rat brain stem resembled that previously reported for the whole brain; synaptosomes sedimented in a sucrose gradient in subfractions C and D. In the 1-day old rat, mature synaptosomes were found in subfractions A, B, C and D; E contained mainly free mitochondria. 80–95% of the processes in the adult and 10–30% in the 1-day old rat contained synaptic vesicles which were of four types: (1) small agranular vesicles (2) large dense core vesicles (3) large agranular vesicles (4) coated vesicles. Pre- and postsynaptic membrane thickenings were demonstrated in many nerve-ending particles. In the subfractions of the 1-day old rat the protein content was one half and the distribution resembled that in the adult. Evidently nerve endings develop faster in the brain stem than in cortical areas; a serotoninor adrenergic origin of the early synaptosomes is suggested.

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Authors and Affiliations

  1. Department of Anatomy, University of Helsinki, Helsinki, Finland

    Antti Hervonen, Lasse Kanerva, Anja H. Tissari & Birgit V. A. Suurhasko

  2. Department of Pharmacology, University of Helsinki, Helsinki, Finland

    Antti Hervonen, Lasse Kanerva, Anja H. Tissari & Birgit V. A. Suurhasko

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  1. Antti Hervonen
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  2. Lasse Kanerva
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  3. Anja H. Tissari
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  4. Birgit V. A. Suurhasko
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This study was supported by a grant from the Paulo Foundation.

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Hervonen, A., Kanerva, L., Tissari, A.H. et al. Ultrastructure of synaptosomes from one-day old and adult rat brain stem. Cell Tissue Res. 148, 535–550 (1974). https://doi.org/10.1007/BF00221937

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