Abstract
In potato, 11 resistance alleles (R1–R11) are known which confer race-specific resistance to the fungus Phytophthora infestans. R1 has been mapped previously to potato chromosome V and R3 to chromosome XI. Here we report on the localization of the R6 and R7 alleles on the genetic map of potato. Differential resistant strains of tetraploid Solanum tuberosum, clones MaR6 and MaR7, were used as parental plants for the parthenogenetic induction and selection of diploid genotypes containing the R6 or the R7 resistance allele to P. infestans. One resistant dihaploid from MaR7 could be used directly as a parent to produce diploid F1 progeny suitable for phenotypic and RFLP analysis. MaR6 did not produce useful dihaploids directly. After crossing MaR6 with a tetraploid susceptible genotype, resistant F1 clones were selected. The resistant genotypes were then used as parents for the induction of dihaploids. Six dihaploids bearing R6 were identified that could be crossed with a diploid susceptible genotype. Two diploid F1 populations, segregating for R6 and R7, respectively, were analysed with RFLP markers known to be linked with previously identified R genes. Markers linked with R3 were found also to be linked with R6 and R7. The resistance alleles R6 and R7 mapped to a similar distal position on chromosome XI as the R3 allele.
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El-Kharbotly A, Leonards-Schippers C, Huigen DJ, Jacobsen E, Pereira A, Stiekema WJ, Salamini F, Gebhardt C (1994) Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242:749–754
Flipse E, Keetels CJAM, Jacobsen E, Visser RGF (1996) The GBSS-gene has a dosage effect on the starch properties in potato: the influence of amylose on the physico-chemical behaviour of starch. Theor Appl Genet 92:121–127
Frandsen NO (1967) Chimären verschiedener Ploidiestufen aus haploiden Kartoffelklonen. Z Pflanzenzucht 57:123–145
Gebhardt C (1994) RFLP mapping in potato of qualitative and quantitative genetic loci conferring resistance to potato pathogens. Am Potato J 71:339–345
Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75
Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with homoeologous tomato genome. Theor Appl Genet 83:49–57
Gebhardt C, Ritter E, Salamini F (1994) RFLP map of the potato. In Vasil IK, Philips RL (eds) Advances in cellular and molecular biology of plants, Vol I: DNA-based markers in plants. Kluwer Academic Publishers, Dordrecht Boston London, pp 271–285
Hermsen JGT, Verdenius J (1973) Selection from Solanum tuberosum group phureja of genotypes combining high-frequency haploid induction with homozygosity for embryo spot. Euphytica 22:244–259
Hutten RCB, Scholberg EJMM, Huigen DJ, Hermsen JGT, Jacobsen E (1994) Analysis of dihaploid induction and production ability and seed parent x pollinator interaction in potato. Euphytica 72:61–64
Jacobsen E, Hovenkamp-Hermelink JHM, Krijgsheld HT, Nijdam H, Pijnacker LP, Witholt B, Feenstra WJ (1989) Phenotypic and genotypic characterization of an amylose-free starch mutant of potato. Euphytica 44:43–48
Leonards-Schippers C, Gieffers W, Salamini F, Gebhardt C (1992) The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet 233:278–283
Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C (1994) Quantitative resistance to Phytophthora infestans in potato: a case study of QTL mapping in an allogamous plant species. Genetics 137:67–77
Malcolmson JF, Black W (1966) New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica 15:199–203
Mastenbroek C (1953) Experiments on the inheritance of blight immunity in potatoes derived from Solanum demissum Lindl. Euphytica 2:197–206
Mindrinos M, Katagiri F, Yu G-L, Ausubel FM (1994) The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats. Cell 78:1089–1099
Pryor T (1987) The origin and structure of fungal disease resistance genes in plants. Trends Genet 3:157–161
Segal G, Sarfatti M, Schaffer MA, Ori M, Zamir D, Fluhr R (1992) Correlation of genetic and physical structure in the region surrounding the I2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231:179–185
Shaw DS (1991) Genetics. In: Ingram DS, Williams PH (eds) Advances in Plant Pathology, vol 7: Phytophthora infestans, the cause of late blight of potato. Academic Press, London San Diego New York Sydney Tokyo Toronto, pp 131–170
Umaerus V, Umaerus M, Erjefält L, Nilsson BA (1983) Control of Phytophthora by host resistances: problems and progress. In: Erwin DC, Bartnicki-Garcia S, Tsao PH (eds) Phytophthora, its biology, taxonomy, ecology and pathology. Am Phytopath Soc, Saint Paul, Minnesota, USA, pp 315–326
Wastie RL (1991) Breeding for resistance. In: Ingram DS, Williams PH (eds) Advances in Plant Pathology, vol 7: Phytophthora infestans, the cause of late blight of potato. Academic Press, London San Diego New York Sydney Tokyo Toronto, pp 193–223
Witham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C, Baker B (1994) The product of the tobacco mosaic virus resistance gene N: similarity to Toll and the interleukin-1 receptor. Cell 78:1101–1115
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El-Kharbotly, A., Palomino-Sánchez, C., Salamini, F. et al. R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI. Theoret. Appl. Genetics 92, 880–884 (1996). https://doi.org/10.1007/BF00221901
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DOI: https://doi.org/10.1007/BF00221901