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Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts

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Summary

Protoplasts isolated from a barley cell suspension (cv Dissa) were transformed with plasmid DNA containing the neomycinphosphotransferase II (NPT) and β-glucuronidase (GUS) genes, using polyethyleneglycol (PEG) to induce DNA uptake. Transformed microcalli were selected in media containing G418 sulphate. NPT activity was detected in all antibiotic-resistant cell lines, but not all NPT-positive cell lines had GUS activity. Southern analysis confirmed the presence of sequences homologous to the APT and GUS genes in DNA of G418-resistant callus.

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Authors and Affiliations

  1. Institute for General Botany (AMP II), University of Hamburg, Ohnhorststrasse 18, 52, Hamburg, FRG

    P. A. Lazzeri, R. Brettschneider, R. Lührs & H. Lörz

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  1. P. A. Lazzeri
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  2. R. Brettschneider
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  3. R. Lührs
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  4. H. Lörz
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Communicated by G. Wenzel

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Lazzeri, P.A., Brettschneider, R., Lührs, R. et al. Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts. Theoret. Appl. Genetics 81, 437–444 (1991). https://doi.org/10.1007/BF00219433

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  • DOI: https://doi.org/10.1007/BF00219433

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