Summary
The corticotropin-releasing factor (CRF)-containing neurons were investigated in the brain of the domestic fowl by means of the peroxidase-antiperoxidase technique at the light-microscopic level. The detection of CRF-immunoreactivity was facilitated by silver intensification. CRF-containing perikarya were found in the paraventricular, preoptic and mammillary nuclei of the hypothalamus and in some extrahypothalamic areas (nuclei dorsomedialis and dorsolateralis thalami, nucleus accumbens septi, lobus parolfactorius, periaqueductal gray of the mesencephalon, nucleus oculomotorius ventralis). Immunoreactive nerve fibers and terminals were demonstrated in the external zone of the median eminence and the organum vasculosum of the lamina terminalis. These results indicate that an immunologically demonstrable CRF-neurosecretory system also exists in the avian central nervous system.
References
Bons N (1980) The topography of mesotocin and vasotocin systems in the brain of the domestic mallard and Japanese quail: immunocytochemical identification. Cell Tissue Res 213:37–51
Bugnon C, Fellman D, Gouget A, Cardot J (1982) Corticoliberin in the rat brain: immunocytochemical localization of a novel neuroglandular system. Neurosci Lett 30:25–30
De Roos R, De Roos CL (1964) Effects of mammalian corticotropin and chicken adenohypophyseal extracts on steroid genesis by chicken adrenal tissue in vitro. Gen Comp Endocrinol 4:602–607
Dulin WE (1955) The effects of cortisone on the white leghorn cockerel and capon. Poult Sci 34:73–77
Fischman AJ, Moldow RL (1982) Extrahypothalamic distribution of CRF-like immunoreactivity in the rat brain. Peptides 1:149–153
Frankel AI, Graber JW, Nalbandov AV (1967) The effect of hypothalamus lesions on adrenal function in intact and adenohypophysectomized cockerels. Gen Comp Endocrinol 8:387–396
Gallyas F, Görcs T, Merchenthaler I (1982) High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry. J Histochem Cytochem 30:183–189
Goossens N, Blähser S, Oksche A, Vandesande F, Dierick K (1977) Immunocytochemical investigation of the hypothalamo-neurohypophyseal system in birds. Cell Tissue Res 184:1–13
Józsa R, Scanes CG, Vigh S, Mess B (1979) Functional differentiation of the embryonic chicken pituitary gland studied by immunohistological approach. Gen Comp Endocrinol 39:158–163
Kawata M, Hashimoto K, Takahara J, Sano Y (1982) Immunohistochemical demonstration of the localization of corticotropin releasing factor-containing neurons in the hypothalamus of mammals including primates. Anat Embryol (Berl) 165:303–313
Merchenthaler I, Vigh S, Petrusz P, Schally AV (1982) Immunocytochemical localization of corticotropin-releasing factor (CRF) in the rat brain. Am J Anat 165:385–396
Miller RA (1961) Hypertrophic adrenals and their response to stress after lesion in the median eminence of totally hypophysectomized pigeon. Acta Endokrinol Copenh 37:565–576
Nagra CL, Birnic JG, Baum GJ, Meyer RK (1963) The role of the pituitary in regulating steroid secretion by the avian adrenal. Gen Comp Endocrinol 3:274–280
Newcomer WS (1959) Effect of hypophysectomy on some functional aspects of the adrenal gland of the chicken. Endocrinology 65:133–135
Oksche A, Wilson WO, Farner DS (1964) The hypothalamic neurosecretory system of Coturnix coturnix japonica. Z Zellforsch 61:688–709
Péczely P (1969) Localization of CRF activity in the pigeon's diencephalon. In: Polypeptid hormones. Proc 4 Conf of Hung Soc Endocrinol and Metabolism Budapest, Kossuth Budapest, pp 33–36
Resko JA, Norton HW, Nalbandov AV (1964) Endocrine control of the adrenal in chickens. Endocrinology 75:192–200
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Streit P, Reubi JC (1977) A new and sensitive staining method for axonally transported peroxidase (HRP) in the pigeon visual system. Brain Res 126:530–537
Swanson LW, Sawchenko PE, Rivier J, Vale WW (1983) Organization of ovine corticotropin releasing factor immunoreactive cells and fibers in the rat brain: an immunohistochemical study. Neuroendocrinology 36:165–186
Tienhoven A van, Juhász LP (1962) The chicken telencephalon, diencephalon and mesencephalon in stereotaxic coordinates. J Comp Neurol 118:185–197
Vale W, Spiess J, Rivier C, Rivier J (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and β-endorphin. Science 213:1394–1397
Vigh S, Merchenthaler I, Torres-Aleman I, Sueiras-Diaz J, Coy DV, Carter WH, Petrusz P, Schally AV (1982) Corticotropin-releasing factor (CRF): Immunocytochemical localization and radioimmunoassay (RIA). Life Sci 31:2441–2448
Youngreen OM, Phillips RE (1978) A stereotaxic atlas of the brain of the three-day-old domestic chick. J Comp Neurol 181:567–600
Zamboni L, DeMartino C (1967) Buffered picric acid-formaldehyde: A new rapid fixative for electron microscopy. J Cell Biol 35:148A
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Józsa, R., Vigh, S., Schally, A.V. et al. Localization of corticotropin-releasing factor-containing neurons in the brain of the domestic fowl. Cell Tissue Res. 236, 245–248 (1984). https://doi.org/10.1007/BF00216537
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DOI: https://doi.org/10.1007/BF00216537