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Fibronectin (FN) in hypertrophic scars and keloids

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Summary

Fibronectin (FN) distribution was compared among samples of normal human dermis, hypertrophic scar, keloid, and granulation tissues from deep injuries. Localization was established by use of fibronectin antibodies and the indirect immunofluorescence method. Fresh-frozen tissue was sectioned on a cryostat and examined by epifluorescence. Hypertrophic scar and keloid demonstrated heavy deposition of FN, which conformed to the nodular characteristics of the lesions. Intense localization occurred in granulation tissue over fibroblasts which were stellate and vesiculated, and over small blood vessels. FN-staining was weak in areas over fibroblasts which were more rounded and nonvesiculated. Staining for FN was also minimal over the collagen in normal dermis and the deeper, larger collagen fascicles in the lesions. Fibroblasts cultured from normal dermis, hypertrophic scar, and keloid for 5–6 weeks were intensely stained for FN. Extracellular matrix was heavily positive in cultures from the lesions compared with those from normal dermis.

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References

  • Armstrong PD, Armstrong TM (1981) Immunofluorescent histological studies of the role of fibronectin in the expression of the associative preferences of embryonic tissues. J Cell Sci 51:121–133

    Google Scholar 

  • Blackburn WR, Cosman B (1966) Histological basis of keloid and hypertrophic scar differentiation. Arch Pathol 82:65–71

    Google Scholar 

  • Cohen IK, Diegelmann RF, Keiser HR (1976) Collagen metabolism in keloid and hypertrophic scar. In: Longacre JJ (ed) The ultrastructure of collagen, CC Thomas, Springfield, pp 199–212

    Google Scholar 

  • Crocker DJ, Murad TM, Greer JC (1970) Role of the pericyte in wound healing. An ultrastructural study. Exp Mol Pathol 13:51–65

    Google Scholar 

  • Glucksmann A (1964) Cell turnover in the dermis. In: W Montagna, RE Billingham (eds) Advances in biology of the skin: Wound healing. Pergamon Press, Oxford, 5:76–94

    Google Scholar 

  • Grinnell F, Billingham RE, Burgess L (1981) Distribution of fibronectin during wound healing in vivo. J Invest Dermatol 76:181–189

    Google Scholar 

  • Jaffe EA, Mosher DF (1978) Synthesis of fibronectin by cultured human endothelial cells. J Exp Med 147:1779–1791

    Google Scholar 

  • Kischer CW, Brody GS (1981) Structure of the collagen nodule from hypertrophic scars and keloids. Scanning Elect Microsc, 111:371–376

    Google Scholar 

  • Kischer CW, Shetlar MR (1979) Vascular occlusion in repair and regenerative progresses. 37th Ann Proc Electron Mico Soc Am, Claitor's Publ Div Baton Rouge, 96–97

  • Kischer CW, Theis AC, Chvapil M (1982) Perivascular myofibroblasts and microvascular occlusion in hypertrophic scars and keloids. Human Pathol 13:819–824

    Google Scholar 

  • Kurkinen M, Vaheri JA, Roberts PJ, Stenman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51

    Google Scholar 

  • Mautner V, Hynes RO (1977) Surface distribution of LETS protein in relation to the cytoskeleton of normal and transformed cells. J Cell Biol 75:743–768

    Google Scholar 

  • Ross R, Everett NB, Tyler R (1970) Wound healing and collagen formation VI. The origin of the wound fibroblast studied in parabiosis. J Cell Biol 44:745–754

    Google Scholar 

  • Rudolph R, Guber S, Suzuki M, Woodward M (1977) The life cycle of the myofibroblast. Surg Gyn Obst 145:389–394

    Google Scholar 

  • Shetlar MR, Shetlar CL, Kischer CW (1981) Glycosaminoglycans in granulation tissue and hypertrophic scars. Burns 8:27–31

    Google Scholar 

  • Silver MH, Foidart JM, Pratt RM (1981) Distribution of fibronectin and collagen during mouse limb and palate development. Differentiation 18:141–149

    Google Scholar 

  • Weber K, Braun-Falco O (1973) Ultrastructure of blood vessels in human granulation tissue. Arch Derm Forsch 248:29–44

    Google Scholar 

  • Weiss, Roy E, Reddi AH (1981) Appearance of fibronectin during the differentiation of cartilage, bone and bone marrow. J Cell Biol 88:630–636

    Google Scholar 

  • Yamada KM, Kennedy DW, Kimata K, Pratt RM (1980a) Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments. J Biol Chem 255:6055–6063

    Google Scholar 

  • Yamada KM, Olden K, Hahn LHE (1980b) Cell surface protein and cell interactions. In: The cell surface: Mediator of developmental processes, 38th Symp Soc Dev Biol, Vancouver BC, Academic Press, New York, pp 43–78

    Google Scholar 

  • Yamada SS, Yamada KM, Willingham MC (1980c) Intracellular localization of fibronectin by immunoelectron microscopy. J Histochem Cytochem 28:953–960

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, University of Arizona, College of Medicine, Tucson, Arizona, USA

    C. Ward Kischer Ph.D. & Mary J. C. Hendrix

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  1. C. Ward Kischer Ph.D.
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  2. Mary J. C. Hendrix
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Supported in part by NIH Research Grant 1 R01GM 25159

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Ward Kischer, C., Hendrix, M.J.C. Fibronectin (FN) in hypertrophic scars and keloids. Cell Tissue Res. 231, 29–37 (1983). https://doi.org/10.1007/BF00215771

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  • DOI: https://doi.org/10.1007/BF00215771

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