Abstract
4-Chlorindole-3-acetic acid (4-CI-IAA), an endogenous auxin in certain plant species of Fabaceae, has a higher efficiency in stimulating cell elongation of grass coleoptiles compared with indole-3-acetic acid (IAA), particularly at low concentrations. However, some investigations reported a 1,000-fold discrepancy between growth stimulation and binding affinity of 4-CI-IAA to auxin-binding protein 1 (ABP1) from maize. Here we report binding data of 4-CI-IAA and three alkylated IAA derivatives using purified ABP1 in equilibrium dialysis. There is a clear correlation between the growth-promoting effects and the binding affinity to ABP1 of the different IAA analogues measured by competition of [3H]naphthalene-1-acetic acid binding. Our data are consistent with the hypothesis that ABP1 mediates auxin-induced cell elongation.
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Abbreviations
- ABP1:
-
auxin-binding protein 1
- 4-CI-IAA:
-
4-chloroindole-3-acetic acid
- NAA:
-
naphthalene-1-acetic acid
- ER:
-
endoplasmic reticulum
- IAA:
-
indole-3-acetic acid
- 2-Me-IAA:
-
2-methylindole-3-acetic acid
- 4-Me-IAA:
-
4-methylindole-3-acetic acid
- 4-Et-IAA:
-
4-ethylindole-3-acetic acid
- MES:
-
4-morpholineethanesulfonic acid
- PAA:
-
phenylacetic acid
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Rescher, U., Walther, A., Schiebl, C. et al. In vitro binding affinities of 4-chloro-, 2-methyl-, 4-methyl-, and 4-ethylindoleacetic acid to auxin-binding protein 1 (ABP1) correlate with their growth-stimulating activities. J Plant Growth Regul 15, 1 (1996). https://doi.org/10.1007/BF00213127
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DOI: https://doi.org/10.1007/BF00213127