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Identification and characterization of Chromobacterium viscosum lipase

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Biotechnology Techniques

Abstract

Separation of a commercial preparation of Chromobacterium viscosum by hydrophobic interaction chromatography yields two active fractions, one corresponding to a lipase of 33.0 ± 1.0 kDa by SDS-PAGE and the other to a high molecular weight aggregate (> 250 kDa) of the lipase with some impurities absorbing at 436 nm. Partial disaggregation of this complex occurs on gel filtration chromatography in the presence of 1% (w/v) CHAPS. On gel filtration under non reducing conditions the lipase behaves like a 17 kDa protein; in the presence of a strong denaturant and of a reducing agent a molecular size of 36 kDa is obtained, in accordance with SDS-PAGE results.

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Authors and Affiliations

  1. Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, 1000, Lisboa, Portugal

    M. A. Taipa, P. Moura-Pinto & J. M. S. Cabral

Authors
  1. M. A. Taipa
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  2. P. Moura-Pinto
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  3. J. M. S. Cabral
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Taipa, M.A., Moura-Pinto, P. & Cabral, J.M.S. Identification and characterization of Chromobacterium viscosum lipase. Biotechnol Tech 8, 27–32 (1994). https://doi.org/10.1007/BF00207629

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  • DOI: https://doi.org/10.1007/BF00207629

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