Abstract
Variations in culture feeding protocols were used to optimize the secretion of protein and cellulase in Neocallimastix frontalis EB188. High numbers (2000/ml) of zoospores, culture feeding at 55 h using a 1:3 dilution and cotton cellulose [0.25% (w/v) final] as the carbon source increased secretion. Endoglucanase reached 1.6±0.06 IU/ml, exoglucanase reached 0.032±0.006 IU/ml and β-glucosidase reached 0.874±0.090 IU/ml. Medium containing twice the concentration of non-carbon-source components failed to increase secretions. Gel electrophoresis demonstrated that eleven cellulases were present. Two cellulases were secreted only in stationary cultures. rotein and cellulase secretion in N. frontalis EB188 may be dependent on the dilution of fermentation products.
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Correspondence to: R. E. Calza
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Tsai, K.P., Calza, R.E. Optimization of protein and cellulase secretion in Neocallimastix frontalis EB188. Appl Microbiol Biotechnol 39, 477–482 (1993). https://doi.org/10.1007/BF00205036
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DOI: https://doi.org/10.1007/BF00205036