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Therapy of spontaneous pulmonary metastases of a murine mammary carcinoma with anaerobic corynebacteria

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Summary

Corynebacterium parvum given intravenously or into the tumor 7 or 2 days before surgical removal of primary tumors greatly reduced the number of spontaneous pulmonary metastases of a syngeneic mammary carcinoma of C3Hf/Bu mice. When a single dose of 6000 rads γ-rays was used to eliminate primary tumors the number of lung metastases significantly increased. Administration of C. parvum before irradiation not only prevented this metastasis — facilitating effect of radiation, but also reduced the number of lung nodules below that in amputated mice. Metastatic spread was not altered by postoperative treatment of mice with single doses of C. parvum or C. granulosum. However, significantly more mice had lung metastases if they were given 2 intraperitoneal injections of C. granulosum.

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References

  • Adlam, C., Scott, M. T.: Lympho-reticular stimulatory properties of Corynebacterium parvum and related bacteria. J. Med. Microbiol. 6, 261 (1973)

    Google Scholar 

  • Baldwin, R. W., Price, M. R., Robins, R. A., Lyphocyte-mediated cytotoxicity for rat hepatoma cells is blocked by tumour-specific antigen-antibody complexes. Nature New Biol. 238, 185 (1972)

    Google Scholar 

  • Castro, J. E.: The effect of Corynebacterium parvum on the structure and function on the lymphoid system in mice. Europ. J. Cancer 10, 115 (1974)

    Google Scholar 

  • Castro, J. E., Sadler, T. E.: Combined surgery and Corynebacterium parvum for treatment of a metastasizing tumour in mice. Brit. J. Surgery 62, 22 (1975)

    Google Scholar 

  • Crile, G., Deodhar, S. D.: Role of preoperative irradiation in prolonging concomitant immunity and preventing metastases in mice. Cancer 27, 629 (1971)

    Google Scholar 

  • Currie, G. A., Bagshawe, K. D.: Active immunotherapy with Corynebacterium parvum and chemotherapy in murine fibrosarcoma. Brit. Med. J. 1, 541 (1970)

    CAS  PubMed  Google Scholar 

  • Currie, G. A., Basham, C.: Serum mediated inhibition of the immunological reactions of the patient to his own tumour: a possible role for circulating antigen. Brit. J. Cancer 26, 427 (1972)

    Google Scholar 

  • Fisher, B., Wolmark, N., Saffer, E., Fisher, E. R.: Inhibitory effect of prolonged Corynebacterium parvum and cyclophosphamide administration on the growth of established tumors. Cancer 35, 134 (1975)

    Google Scholar 

  • Likhite, V. V.: Rejection of tumors and metastases in Fischer 344 rats following intratumor administration of killed Corynebacterium parvum. Int. J. Cancer 14, 684 (1974)

    Google Scholar 

  • Likhite, V. V., Halpern, B. N.: Lasting rejection of mammary adenocarcinoma cell tumors in DBA/2 mice with intratumor injection of killed Corynebacterium parvum. Cancer Res. 34, 341 (1974)

    CAS  PubMed  Google Scholar 

  • Milas, L., Gutterman, J. U., Bašić, I., Hunter, N., Mavligit, G., Hersh, E. M., Withers, H. R.: Immunoprophylaxis and immunotherapy for a murine fibrosarcoma with C. granulosum and C. parvum. Int. J. Cancer 14, 493 (1974a)

    CAS  PubMed  Google Scholar 

  • Milas, L., Hunter, N., Bašić, I., Withers, H. R.: Protection by Corynebacterium granulosum against radiation-induced enhancement of artificial pulmonary metastases of a murine fibrosarcoma. J. Natl. Cancer Inst. 52, 1875 (1974b)

    Google Scholar 

  • Milas, L., Bašić, I., Kogelnik, H. D., Withers, H. R.: Effects of Corynebacterium granulosum on weight and histology of lymphoid organs, response to mitogens, skin allografts, and a syngeneic fibrosarcoma in mice. Cancer Res. 35, 2365 (1975a)

    Google Scholar 

  • Milas, L., Hunter, N., Bašić, I., Mason, K., Grdina, D. J., Withers, H. R.: Nonspecific immunotherapy of murine solid tumors with Corynebacterium granulosum. J. Natl. Cancer Inst. 54, 895 (1975b)

    Google Scholar 

  • Milas, L., Hunter, N., Withers, H. R.: Combination of local irradiation with systemic application of anaerobic corynebacteria in therapy of a murine fibrosarcoma. Cancer Res. 35, 1274 (1975c)

    Google Scholar 

  • Milas, L., Kogelnik, H. D., Bašić, I., Mason, K., Hunter, N., Withers, H. R.: Combination of C. parvum and specific immunization against artificial pulmonary metastases in mice. Int. J. Cancer 16, 738 (1975d)

    Google Scholar 

  • Milas, L., Hunter, N., Withers, H. R.: Concomitant immunity to pulmonary metastases of a murine fibrosarcoma: Influence of removal of primary tumor by radiation or surgery, or active immunization and treatment with C. granulosum. Intern. J. Rad. Oncol. Biol. Physics. (in press) 1976

  • Paslin, D., Dimitrov, N. V., Heaton, C.: Regression of a transplantable hamster melanoma by intralesional injection of Corynebacterium granulosum. J. Natl. Cancer Inst. 52, 571 (1974)

    Google Scholar 

  • Proctor, J., Rudenstam, C. M., Alexander, P.: Increased incidence of lung metastases following treatment of rats bearing hepatomas with irradiated tumour cells and the beneficial effect of Corynebacterium parvum in this system. Biomedicine 19, 248 (1973)

    Google Scholar 

  • Scott, M. T.: Corynebacterium parvum as a therapeutic antitumor agent in mice. II. Local injection. J. Natl. Cancer Inst. 53, 861 (1974)

    Google Scholar 

  • Suit, H. D., Kaštelan, A.: Immunologic status of host and response of a methylcholanthrene-induced sarcoma to local X-irradiation. Cancer 26, 232 (1970)

    Google Scholar 

  • Suit, H. D., Sedlacek, R., Wagner, M., Orsi, L.: Radiation response of C3H fibrosarcoma enhanced in mice stimulated by Corynebacterium parvum. Nature 255, 493 (1975)

    Google Scholar 

  • Suit, H. D., Sedlacek, R., Wagner, M., Silobrčić, V., Orsi, L., Rothman, K.: Effect of C. parvum on the response to irradiation of a C3H fibrosarcoma. Cancer Res. 36, 1305 (1976)

    Google Scholar 

  • Tannock, I. F., Suit, H. D., Marshall, N.: Vitamin A and the radiation response of experimental tumors: An immune-mediated effect. J. Natl. Cancer Inst. 48, 731 (1972)

    Google Scholar 

  • Vaage, J.: Specific desensitization of resistance against a syngeneic methylcholanthrene-induced sarcoma in C3H mice. Cancer Res. 32, 193 (1972)

    Google Scholar 

  • Woodruff, M. F. A., Dunbar, N.: The effect of Corynebacterium parvum and other reticulo-endothelial stimulants on transplanted tumours in mice. In: Ciba Foundation Symposium on Immunopotentiation, p. 287. Amsterdam: Associated Scientific 1973

    Google Scholar 

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Authors and Affiliations

  1. Central Institute for Tumors and Allied Diseases, Ilica 197, 41000, Zagreb, Yugoslavia

    L. Milas

  2. Section of Experimental Radiotherapy, University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute, 77025, Houston, Texas, USA

    K. Mason & H. R. Withers

Authors
  1. L. Milas
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  2. K. Mason
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  3. H. R. Withers
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Milas, L., Mason, K. & Withers, H.R. Therapy of spontaneous pulmonary metastases of a murine mammary carcinoma with anaerobic corynebacteria. Cancer Immunol Immunother 1, 233–237 (1976). https://doi.org/10.1007/BF00200098

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  • DOI: https://doi.org/10.1007/BF00200098

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