Abstract
The occurrence of glycolate oxidase in addition to glycolate dehydrogenase in Dunaliella salina and D. primolecta, as reported in the literature, could not be confirmed. Both species were demonstrated to possess only glycolate dehydrogenase. After separation of organelles by gradient centrifugation, glycolate dehydrogenase along with hydroxypyruvate reductase was found exclusively in the mitochondria. Thus the peroxisomes from Dunaliella are not of the leaf-type: because of their content of catalase, uricase and hydroxyacyl-CoA dehydrogenase they appear to be of the same type as in Eremosphaera and other chlorophycean algae. No activity of glycolate dehydrogenase was found in the chloroplast fraction when the 2,6-dichlorophenol-indophenol test was used.
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This work was supported by the Deutsche Forschungsgemeinschaft.
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Stabenau, H., Winkler, U. & Säftel, W. Localization of glycolate dehydrogenase in two species of Dunaliella . Planta 191, 362–364 (1993). https://doi.org/10.1007/BF00195694
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DOI: https://doi.org/10.1007/BF00195694