Abstract
The electrofusion-mediated fertilization of single egg cells of wheat with isolated individually selected wheat sperm cells was successfully carried out for the first time. On average the fusion frequency was 30% but under optimal conditions it was possible to reach as much as 55%. Two days after electric fusion 60% of the fusion products started to divide, 88.5% of them forming multicellular structures and in a few cases microcalluses. The culture of single unfertilized egg cells with or without the application of AC field and electric pulses induced no cell division. The egg cells and fusion products were cultured in a maize feeder-cell system.
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Abbreviations
- AC:
-
Alternating current
- DC:
-
Direct current
- DAPI 4′,6:
-
diamino-2-phenylindole
References
Coleman AW, Goff LJ (1985) Stain Technol 60:145–154
Dumas C, Russell SD (1992) Int Rev Cytol 140:565–592
Holm PB, Knudsen S, Mouritzen P, Negri D, Olsen FL, Roué C (1994) The Plant Cell 6:531–543
Huang B-Q, Russell SD (1992) Int Rev Cytol 140:233–293
Jensen WA (1974) In: Dynamic Aspects of Plant Ultrastructure, A.W. Robards (ed) New York: McGraw Hill, pp. 481–503.
Kovács M, Barnabás B, Kranz E (1994) Sex Plant Reprod 7. 5:311–312
Kranz E, Bautor J, Lörz H (1991a) Sex Plant Reprod 4:12–16
Kranz E, Bautor J, Lörz B (1991b) Sex Plant Reprod 4:17–21
Kranz E, Lörz H, Diogenet C, Faure J-E (1992) Int Rev Cytol 140:407–423
Kranz E and Lörz H (1993) The Plant Cell 5:739–746
Mogensen LH (1982) Carlsberg Res Commun 47:313–354
Murashige T, Skoog F (1962) Physiol Plant 15:137–160
Russel SD (1983) Am J Bot 70:416–434
Russel SD (1992) Int Rev Cytol 140:357–388
Russel SD (1993) The Plant Cell 5:1349–1359
Theunis CH, Pierson ES, Cresti M (1991) Sex Plant Reprod 4:145–154
van Went JL, Willemse MTM (1984) In: Embryology of Angiosperms, BM Johri (ed) Berlin: Springer-Verlag, pp. 273–317.
Yang H-Y, Zhou C (1992) Am J Bot 79:354–363
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Communicated by M. R. Davey
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Kovács, M., Barnabás, B. & Kranz, E. Electro-fused isolated wheat (Triticum aestivum L.) gametes develop into multicellular structures. Plant Cell Reports 15, 178–180 (1995). https://doi.org/10.1007/BF00193715
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DOI: https://doi.org/10.1007/BF00193715