Abstract
Laser-velocimetry was applied in order to study the effect of light on the velocity of protoplasmic streaming (pps) in Characean cells. A change from dark to light (= 6 W · m−2) leads to an acceleration of streaming by about 15–30% with a time-constant of approx. 300 s. The transition from light to dark causes a transient decrease of velocity below the original dark level. This response occurs with a time constant of about 500 s. It returns to its initial value with a time-constant of about 2000 s. This may indicate that a control loop of cytosolic homeostasis takes a decrease in pCa more seriously than an increase. A possible involvement of temperature effects caused by illumination was excluded by measuring the influence of temperature. Steady-state velocity of streaming changed by 5% per 1° C. Irradiation with infra-red light (λ > 780 nm) did not cause a change in velocity. The absence of a light effect on streaming velocity in the presence of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU) shows that photosynthesis and not phytochrome is involved. The role of light-induced changes of pCa is discussed, especially with respect to the hypothesis of Vanselow and Hansen (1989, J. Membr. Biol. 110, 175–187) that photosynthesis acts on the plasmalemma K+-channel via light-induced uptake of Ca2+ into the chloroplasts.
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Abbreviations
- ASF:
-
auto structure function
- DCMU:
-
3-(3′,4′-dichlorophenyl)-1,1-dimethylurea
- pps:
-
protoplasmic streaming
- τL, τD, τC :
-
time-constants of the light and dark responses, and of a putative Ca-control system
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Financial support by the Deutsche Forschungsgemeinschaft is gratefully acknowledged. The first author was granted a scholarship by the state of Schleswig-Holstein. We are indebted to Prof. Dr. G. Pfister for technical advice and helpful discussions and to Mrs. E. Götting for drawing the figures.
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Plieth, C., Hansen, UP. Light dependence of protoplasmic streaming in Nitella flexilis L. as measured by means of laser-velocimetry. Planta 188, 332–339 (1992). https://doi.org/10.1007/BF00192799
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DOI: https://doi.org/10.1007/BF00192799