Abstract
High titered Clostridium sordellii lethal toxin antiserum, cross-reactive with C. difficile cytotoxin B (ToxB), was used to isolate toxB fragments from a C. difficile expression library. Recombinant clones containing toxB fragments of the 5′ and 3′ end were isolate. A 2.5-kb HincII fragment of chromosomal DNA overlaps both groups of clones. A partial restriction map of the total toxB gene is presented. The gene is positioned upstream of utxA and toxA toxB has a size of 6.9kb, corresponding to a 250-kDa polypeptide. A partial sequence of the 5′ end of toxB was determined. The sequence contains 398 bp upstream of toxB with a putative Shine-Dalgarno box (AGGAGA) and 609 bp of the toxB open reading frame. The N-terminal 203 amino acids of ToxB were compared with the N-terminal amino acids of the enterotoxin A (ToxA). A homology of 64% of the residues was detected, which proves the relatedness of ToxA and ToxB of C. difficile.
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Abbreviations
- PBS:
-
phosphate-buffered saline
- ToxA:
-
C. difficile enterotoxin A
- ToxB:
-
C. difficile cytotoxin B
- toxB :
-
gene encoding ToxB
- HT:
-
C. sordellii hemorrhagic toxin
- LT:
-
C. sordellii lethal toxin
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von Eichel-Streiber, C., Laufenberg-Feldmann, R., Sartingen, S. et al. Cloning of Clostridium difficile toxin B gene and demonstration of high N-terminal homology between toxin A and B. Med Microbiol Immunol 179, 271–279 (1990). https://doi.org/10.1007/BF00192465
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DOI: https://doi.org/10.1007/BF00192465