Bergmann glial development in the mouse cerebellum as revealed by tenascin expression

Abstract

Tenascin, an astroglia-derived extracellular matrix molecule, is also expressed by radial glia of the embryonic mouse cerebellum. Expression of tenascin can thus be applied as a marker of astroglial development from an early stage, especially prior to the expression of the glial fibrillary acidic protein (GFAP) that can be detected in the postnatal cerebellum. The development of Bergmann glia, specialized cerebello-cortical astroglia with radial processes, was examined by tenascin immunohistochemistry and non-radioactive in situ hybridization histochemistry for tenascin mRNA in the developing mouse cerebellum. Tenascin-immunopositive radial glial processes extending from the ventricular zone to the pia mater retracted toward the cortex in the embryonic cerebellum and occupied a position corresponding to the Bergmann glial processes at the perinatal stage. Tenascin gene-expressing cells were generated in the ventricular zone of the cerbellar primordium and migrated radially toward the cortex. They were stratified in the layer of Bergmann glial somata at the early postnatal stage. They extended GFAP-immunopositive radial processes from the somata to the pia mater as revealed by double-labeling employing tenascin in situ hybridization histochemistry and GFAP-immunostaining. Bergmann glia are therefore considered to develop from cerebellar radial glia by migration of their somata and retraction of their processes. The tenascin gene-expressing cells displayed mitotic activity after alignment in the cortex as revealed by double-labeling by tenascin in situ hybridization histochemistry and immunohistochemical detection of the incorporated bromodeoxyuridine. The above findings suggest that the Bergmann glia in the cortex represent one of the origins of the astroglia in the developing cerebellum.