Skip to main content
SpringerLink
Log in

Production of androsta-1,4-diene-3,17-dione from cholesterol using immobilized growing cells of Mycobacterium sp. NRRL B-3683 adsorbed on solid carriers

  • Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

Living cells of Mycobacterium sp. NRRL B-3683 were immobilized by adsorption on different types of solid carriers in order to produce androsta-1,4-diene-3,17-dione (ADD) from cholesterol. Activated alumina proved to be the most preferred carrier for long-term operation when glucose and peptone were added to the reaction medium. In a repeated-batch process, the maximum productivity of ADD was about 0.19 g/l per day with a molar conversion rate of 77% when 1.0 g/l of cholesterol was added to the reaction medium. The half-life of the immobilized cells was more than 45 days and the system could be reactivated by incubating the immobilized cells in a cell growth medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Buchs J, Mozes N, Wandrey C, Rouxhet PG (1988) Cell adsorption control by culture conditions: influence of phosphate on surface properties, flocculation and adsorption behaviour of Corynebacterium glutamicum. Appl Microbiol Biotechnol 29:119–128

    Google Scholar 

  • Buckland BC, Dunnill P, Lilly MD (1975) The enzymatic transformation of water-insoluble reactants in nonaqueous solvents: conversion of cholesterol to cholest-4-ene-3-one by a Nocardia sp. Biotechnol Bioeng 17:815–826

    Google Scholar 

  • Dighe AS, Patel PM, Rao KK (1985) Effect of phosphate on the estimation of reducing sugars in biological media. Biotechnol Bioeng 27:1612–1615

    Google Scholar 

  • Duarte JMC, Lilly MD (1984) Cholesterol degradation by polymer-entrapped Nocardia in organic solvents. Enzym Eng 7:573–576

    Google Scholar 

  • El-Aassar SA, Omar SH, Rehm HJ (1988) Oxidation of n-tetradecane by Candida parapsilosis KSh 21 adsorbed on different glass rings. Appl Microbiol Biotechnol 29:442–446

    Google Scholar 

  • Flygare S, Larsson PO (1987) Steroid transformation using magnetically immobilized Mycobacterium sp. Enzyme Microb Technol 9:494–499

    Google Scholar 

  • Flygare S, Larsson PO (1989) Steroid transformation in aqueous two-phase systems: side-chain degradation of cholesterol by Mycobacterium sp. Enzyme Microb Technol 11:752–759

    Google Scholar 

  • Fukui S, Tanaka A (1982) Immobilized microbial cells. Ann Rev Microbiol 36:145–172

    Google Scholar 

  • Haberland ME, Reynolds JA (1973) Self-association of cholesterol in aqueous solution. Proc Nat Acad Sci USA 70:2313–2316

    Google Scholar 

  • Imada Y, Ishikawa H, Nishikawa (1981) Development of new steroid fermentation. Nippon Nogeikagaku Kaishi 55:713–721

    Google Scholar 

  • Kolot FB (1982) Microbial catalysts for steroid transformation: part 1. Process Biochem 17 (6):12–18

    Google Scholar 

  • Kolot FB (1983) Microbial catalysts for steroid transformation: part 2. Process Biochem 18(1):19–21

    Google Scholar 

  • Koscheyenko KA, Turkina MV, Skryabin GK (1983) Immobilization of living microbial cells and their application for steroid transformation. Enzyme Microb Technol 5:14–21

    Google Scholar 

  • Lee CY, Liu WH (1990) Microbial transformation of cholesterol to androsta-1,4-diene-3,17-dione by kappa-carrageenan-entrapped cells of Mycobacterium sp. NRRL B-3683. J Chin Agric Chem Soc 28:276–284

    Google Scholar 

  • Lenz GR (1983) Steroids. In: Brown J, Eastman CI, Golojuch C, Klingsberg A, Wainwright M (eds) Kirk-Othmer Encyclopedia of Chemical Technology, 3rd edn, vol 21. Wiley, New York, p 645

    Google Scholar 

  • Liu WH, Lee CY (1990) Microbial transformation of cholesterol to androsta-1,4-diene-3,17-dione by Mycobacterium sp. NRRL B-3683 in a cholesterol-synthetic medium. J Chin Agric Chem Soc 28:166–174

    Google Scholar 

  • Marsheck WJ, Kraychy S, Muir R (1972) Microbial degradation of sterols. Appl Microbiol 23:72–77

    Google Scholar 

  • Mozes N, Rouxhet PG (1984) Dehydrogenation of cortisol by Arthrobacter simplex immobilized as supported monolayer. Enzyme Microb Technol 6:497–502

    Google Scholar 

  • Mozes N, Rouxhet PG (1985) Metabolic activity of yeast immobilized as supported monolayer. Appl Microbiol Biotechnol 22:92–97

    Google Scholar 

  • Oshima T, Kimura F, Komata T, Iwamoto Y (1984) Method for the fermentative production of androsta-1,4-diene-3,17-dione. Japanese patent publication SHO59-37078

  • Jr Wingard LB, Roach RP, Miyawaki O, Egler KA, Klinzing GE, Silver RS, Brackin JS (1985) Epoxidation of propylene utilizing Nocardia corallina immobilized by gel entrapment or adsorption. Enzyme Microb Technol 7:503–509

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China

    Chung-Yi Lee & Wen-Hsiung Liu

  2. Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China

    Chung-Yi Lee

Authors
  1. Chung-Yi Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wen-Hsiung Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, CY., Liu, WH. Production of androsta-1,4-diene-3,17-dione from cholesterol using immobilized growing cells of Mycobacterium sp. NRRL B-3683 adsorbed on solid carriers. Appl Microbiol Biotechnol 36, 598–603 (1992). https://doi.org/10.1007/BF00183235

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00183235

Keywords

Search

Navigation