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Improved stability of the lipase from Candida rugosa in different purification degrees by chemical modification

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Biotechnology Techniques

Summary

Semipurified lipase of Candida rugosa and pure isoforms (lipase A and lipase B) have been chemically modified using two methodologies based on polyethyleneglycol (PEG). The activation of PEG with p-NO2-phenylchloroformate gives better biocatalysts than those obtained with cyanuric chloride-PEG in the enzymatic activity of the lipase. The chemical modification increases the stability of pure lipases in isooctane at 50 °C.

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Authors and Affiliations

  1. Department of Organic and Pharmaceutical Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040, Madrid, Spain

    M. J. Hernáiz, J. M. Sánchez-Montero & J. V. Sinisterra

Authors
  1. M. J. Hernáiz
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  2. J. M. Sánchez-Montero
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  3. J. V. Sinisterra
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Hernáiz, M.J., Sánchez-Montero, J.M. & Sinisterra, J.V. Improved stability of the lipase from Candida rugosa in different purification degrees by chemical modification. Biotechnol Tech 10, 917–922 (1996). https://doi.org/10.1007/BF00180394

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  • DOI: https://doi.org/10.1007/BF00180394

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